Lopes Alessandra B, Lopes Larissa B, da Silveira Antunes Roseli N, Fukasawa Josianne T, de A Cavaretto Débora, Calamita Zamir
Postgraduate Program in Health and Aging, Marilia School of Medicine - FAMEMA, Rua Monte Carmelo, 800, Fragata, CEP: 17519-030, Marilia - SP, Brazil.
Discipline of Hematology and Hemotherapy of the Federal University of Sao Paulo - UNIFESP, Rua Doutor Diogo de Faria, 824, Vila Clementino, CEP: 04037-002, Sao Paulo - SP, Brazil.
Curr Aging Sci. 2018;11(2):118-125. doi: 10.2174/1874609811666180605092234.
Immunosenescence is a remodeling of the immune system, caused by aging, with changes in the function of neutrophils, lymphocytes, and Treg cells.
This study aimed to evaluate the expression of molecules CD11b, CD16 and CD64 (neutrophils), CD154 (T lymphocytes), CD40 (B lymphocytes), and to quantitatively analyze the Treg cell subpopulation.
49 elderlies (≥60 years) and 49 adults (≤35 years) were studied. Flow cytometry was used to analyze the expression of surface molecules and the subpopulation of Treg cells, and the results between the groups were compared statistically by the t-test.
There was a decreased significance in the expression of CD11b and CD40 in the elderly.
Decreased CD11b expression can result in susceptibility to infectious diseases, and impairment of phagocytic capacity. Decreased CD40 expression can result in a decline in B lymphocyte activation. The other molecule studied presented alterations not significant, but compatible with the immunological changes in aging.
免疫衰老指免疫系统随年龄增长而重塑，中性粒细胞、淋巴细胞及调节性T细胞功能发生改变。
本研究旨在评估分子CD11b、CD16和CD64（中性粒细胞）、CD154（T淋巴细胞）、CD40（B淋巴细胞）的表达，并对调节性T细胞亚群进行定量分析。
对49名老年人（≥60岁）和49名成年人（≤35岁）进行研究。采用流式细胞术分析表面分子表达及调节性T细胞亚群，组间结果采用t检验进行统计学比较。
老年人中CD11b和CD40的表达有显著下降。
CD11b表达降低可导致对传染病的易感性增加及吞噬能力受损。CD40表达降低可导致B淋巴细胞活化下降。所研究的其他分子虽有改变但不显著，不过与衰老过程中的免疫变化相符。
