Vereshchaka Inna V, Bulgakova Nataliya V, Maznychenko Andriy V, Gonchar Olga O, Prylutskyy Yuriy I, Ritter Uwe, Moska Waldemar, Tomiak Tomasz, Nozdrenko Dmytro M, Mishchenko Iryna V, Kostyukov Alexander I
The Unit of the Theory of Physical Education, The Chair of Physical Education, Gdansk University of Physical Education and Sport Gdańsk, Poland.
Department of Movement Physiology, Bogomoletz Institute of Physiology, National Academy of Sciences, Kyiv, Ukraine.
Front Physiol. 2018 May 15;9:517. doi: 10.3389/fphys.2018.00517. eCollection 2018.
The aim of this study is to detect the effects of C fullerenes, which possess pronounced antioxidant properties, in comparison with the actions of the known exogenous antioxidants -acetylcysteine (NAC) and β-Alanine in terms of exercise tolerance and contractile property changes of the () during development of the muscle fatigue in rats. The electrical stimulation of the muscle during four 30 min series in control rats led to total reduction of the muscle contraction force. Furthermore, the effects of prior intraperitoneal (i.p.) or oral CFAS application and preliminary i.p. injection of NAC or β-Alanine on muscle contraction force under fatigue development conditions is studied. In contrast to control rats, animals with CFAS, NAC, or β-Alanine administration could maintain a constant level of muscle effort over five stimulation series. The accumulation of secondary products and changes in antioxidant levels in the muscle tissues were also determined after the fatigue tests. The increased levels of lactic acid, thiobarbituric acid reactive substances and HO after stimulation were statistically significant with respect to intact muscles. In the working muscle, there was a significant ( < 0.05) increase in the activity of endogenous antioxidants: reduced glutathione, catalase, glutathione peroxidase, and superoxide dismutase. Treated animal groups showed a decrease in endogenous antioxidant activity relative to the fatigue-induced animals ( < 0.05). Oral CFAS administration clearly demonstrated an action on skeletal muscle fatigue development similar to the effects of i.p. injections of the exogenous antioxidants NAC or β-Alanine. This creates opportunities to oral use of CFAS as a potential therapeutic agent. Due to the membranotropic activity of C fullerenes, non-toxic CFAS has a more pronounced effect on the prooxidant-antioxidant homeostasis of muscle tissues in rats.
本研究的目的是检测具有显著抗氧化特性的C富勒烯的效果，并将其与已知的外源性抗氧化剂——乙酰半胱氨酸（NAC）和β-丙氨酸在大鼠肌肉疲劳发展过程中的运动耐力和收缩特性变化方面的作用进行比较。在对照大鼠中，对肌肉进行四个30分钟系列的电刺激导致肌肉收缩力完全降低。此外，还研究了预先腹腔内（i.p.）或口服CFAS以及预先腹腔内注射NAC或β-丙氨酸对疲劳发展条件下肌肉收缩力的影响。与对照大鼠相比，给予CFAS、NAC或β-丙氨酸的动物在五个刺激系列中能够保持恒定的肌肉用力水平。疲劳试验后还测定了肌肉组织中次级产物的积累和抗氧化剂水平的变化。刺激后乳酸、硫代巴比妥酸反应性物质和HO水平的升高相对于完整肌肉具有统计学意义。在工作肌肉中，内源性抗氧化剂（还原型谷胱甘肽、过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶）的活性显著（<0.05）增加。与疲劳诱导的动物相比，处理过的动物组内源性抗氧化活性降低（<0.05）。口服CFAS的作用明显表明其对骨骼肌疲劳发展的影响类似于腹腔内注射外源性抗氧化剂NAC或β-丙氨酸的效果。这为口服使用CFAS作为潜在治疗剂创造了机会。由于C富勒烯的膜otropic活性，无毒的CFAS对大鼠肌肉组织的促氧化剂-抗氧化剂稳态具有更显著的影响。
