Weirick Tyler, Militello Giuseppe, Uchida Shizuka
Cardiovascular Innovation Institute, University of Louisville, Louisville, KY, United States.
Front Physiol. 2018 May 14;9:522. doi: 10.3389/fphys.2018.00522. eCollection 2018.
In recent years, the role of RNA has expanded to the extent that protein-coding RNAs are now the minority with a variety of non-coding RNAs (ncRNAs) now comprising the majority of RNAs in higher organisms. A major contributor to this shift in understanding is RNA sequencing (RNA-seq), which allows a largely unconstrained method for monitoring the status of RNA from whole organisms down to a single cell. This observational power presents both challenges and new opportunities, which require specialized bioinformatics tools to extract knowledge from the data and the ability to reuse data for multiple studies. In this review, we summarize the current status of long non-coding RNA (lncRNA) research in endothelial biology. Then, we will cover computational methods for identifying, annotating, and characterizing lncRNAs in the heart, especially endothelial cells.
近年来，RNA的作用已得到扩展，以至于蛋白质编码RNA如今已占少数，而多种非编码RNA（ncRNA）现在构成了高等生物中RNA的大部分。这种认知转变的一个主要促成因素是RNA测序（RNA-seq），它提供了一种基本上不受限制的方法，用于监测从整个生物体到单个细胞的RNA状态。这种观察能力既带来了挑战，也带来了新的机遇，这需要专门的生物信息学工具从数据中提取知识，以及将数据用于多项研究的能力。在这篇综述中，我们总结了长链非编码RNA（lncRNA）在内皮生物学研究中的现状。然后，我们将介绍用于识别、注释和表征心脏（尤其是内皮细胞）中lncRNA的计算方法。
