Panara Virginia, Monteiro Rui, Koltowska Katarzyna
Immunology Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.
Front Cell Dev Biol. 2022 May 9;10:891538. doi: 10.3389/fcell.2022.891538. eCollection 2022.
Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of -regulatory elements by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as and . In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the -regulatory sequences that shape the zebrafish vascular network.
表观遗传调控在协调基因表达的时空调控中不可或缺，而基因表达的时空调控是组织发育的基础。评估全基因组表观遗传修饰的新工具的出现，推动了斑马鱼血管生物学领域的重大进展。斑马鱼是一种强大的模型，通过将ATAC-seq、ChIP-seq和CUT&Tag等技术与转基因品系的生成和活体成像相结合，来研究调控元件的活性，以验证这些调控元件的活性。最近，这种方法导致了重要血管基因（如……和……）的关键增强子的鉴定和表征。在这篇综述中，我们将讨论表观遗传学的最新技术如何在斑马鱼中用于确定染色质状态，并评估塑造斑马鱼血管网络的调控序列的功能。
