Jin Yunyun, Yang Qing, Gao Jiayang, Tang Qi, Duan Bo, Yu Ting, Qi Xinglei, Liu Jiming, Wang Rongmin, Dang Ruihua, Lei Chuzhao, Chen Hong, Lan Xianyong
Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Xianan Cattle Technology Development Company, Biyang, 463700, Henan, People's Republic of China.
Biochem Genet. 2018 Dec;56(6):663-676. doi: 10.1007/s10528-018-9868-3. Epub 2018 Jun 4.
Growth traits are complex quantitative traits controlled by numerous candidate genes, and they can be well-evaluated using body measurement traits. As the members of the nicotinamide adenine dinucleotide-dependent family of histone deacetylases, class I sirtuin genes (including SIRT1, SIRT2 and SIRT3) play crucial roles in regulating lipid metabolism, cellular growth and metabolism, suggesting that they are potential candidate genes affecting body measurement traits in animals. Hence, the objective of this work aimed to detect novel insertions/deletions (indels) of SIRT1, SIRT2 and SIRT3 genes in 955 cattle belonging to five breeds, as well as to evaluate their effects on body measurement traits. Herein, the novel 12-bp indel of SIRT1 gene, the 7-bp indel of SIRT2 gene and the 26-bp indel of SIRT3 gene were firstly reported, respectively. The association analysis indicated that the indels within SIRT1 and SIRT2 genes were significantly associated with body measurement traits such as body weight, chest circumference, height at hip cross, hip width, body height, etc. (P < 0.05 or P < 0.01). Therefore, based on these findings, the two novel indel variants within bovine SIRT1 and SIRT2 genes could be considered as potential molecular markers for growth traits in cattle selection practices and breeding.
生长性状是由众多候选基因控制的复杂数量性状，并且可以使用体尺性状进行很好的评估。作为组蛋白去乙酰化酶的烟酰胺腺嘌呤二核苷酸依赖性家族成员，Ⅰ类沉默信息调节因子基因（包括SIRT1、SIRT2和SIRT3）在调节脂质代谢、细胞生长和代谢中起关键作用，这表明它们是影响动物体尺性状的潜在候选基因。因此，本研究的目的是检测955头分属于五个品种的牛中SIRT1、SIRT2和SIRT3基因的新型插入/缺失（indel），并评估它们对体尺性状的影响。在此，首次分别报道了SIRT1基因的新型12bp indel、SIRT2基因的7bp indel和SIRT3基因的26bp indel。关联分析表明，SIRT1和SIRT2基因内的indel与体重、胸围、十字部高度、臀宽、体高等体尺性状显著相关（P<0.05或P<0.01）。因此，基于这些发现，牛SIRT1和SIRT2基因内的两个新型indel变异体可被视为牛选择实践和育种中生长性状的潜在分子标记。
