Gui Linsheng, Hao Ruijie, Zhang Yaran, Zhao Xianlin, Zan Linsen
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center of Northwest A & F University, Yangling, Shaanxi 712100, PR China.
Mol Cell Probes. 2015 Jun;29(3):167-71. doi: 10.1016/j.mcp.2015.03.007. Epub 2015 Mar 31.
Class I sirtuin genes including SIRT1, SIRT2 and SIRT3, are members of the nicotinamide adenine dinucleotide (NAD)-dependent family of histone deacetylases, and play essential roles in senescence, metabolism, and apoptosis. This study was conducted to detect potential polymorphisms of the bovine class I sirtuin genes and explore their relationships with ultrasound carcass traits in Qinchuan cattle. Four non-coding mutations in the 3'UTR (SIRT1: g.25751A > C, SIRT1: g.25846A > G, SIRT2: g.19676G > A and SIRT3: g. 25702C > T) and three mutations in exons (SIRT2: g.4062C > T; SIRT2: g.4406C > T and SIRT3: g.25557A > G) were identified in 468 individuals of Qinchuan cattle. Chi-square tests showed that g.25751A > C, g.19676G > A, and g.25702C > T were in Hardy-Weinberg disequilibrium (χ(2) < χ0.05(2)). The statistical analyses indicated that six SNPs were significantly associated with the ultrasound carcass traits (P < 0.05) except g.4062C > T (SIRT2) (P > 0.05). These results indicate that the variations in the class I sirtuin genes and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
Ⅰ类沉默信息调节因子基因包括SIRT1、SIRT2和SIRT3，是烟酰胺腺嘌呤二核苷酸（NAD）依赖性组蛋白脱乙酰酶家族成员，在衰老、代谢和细胞凋亡中发挥重要作用。本研究旨在检测秦川牛Ⅰ类沉默信息调节因子基因的潜在多态性，并探讨其与超声胴体性状的关系。在468头秦川牛个体中，鉴定出3'非翻译区的4个非编码突变（SIRT1：g.25751A>C、SIRT1：g.25846A>G、SIRT2：g.19676G>A和SIRT3：g.25702C>T）以及外显子中的3个突变（SIRT2：g.4062C>T；SIRT2：g.4406C>T和SIRT3：g.25557A>G）。卡方检验表明，g.25751A>C、g.19676G>A和g.25702C>T处于哈迪-温伯格不平衡状态（χ(2)<χ0.05(2)）。统计分析表明，除g.4062C>T（SIRT2）（P>0.05）外，6个单核苷酸多态性与超声胴体性状显著相关（P<0.05）。这些结果表明，Ⅰ类沉默信息调节因子基因的变异及其相应基因型可被视为牛育种经济性状的分子标记。
