Soto Carissa M, Dressick Walter J
Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC, USA.
Materials and Applications Division, US Bureau of Engraving and Printing, Washington, DC, USA.
Methods Mol Biol. 2018;1776:533-552. doi: 10.1007/978-1-4939-7808-3_34.
Metallic nanoscale 3D architectures concentrate electromagnetic energy at precise spatial locations to enable sensing and photocatalysis applications. We have developed solution-based methods to reproducibly fabricate 3D gold nanostructures useful as efficient surface-enhanced Raman spectroscopy (SERS) biosensors. Virus capsids were recruited as templates to assemble gold nanoparticles on their surfaces at well-defined locations to prepare the nanoscale 3D structures. Cowpea mosaic virus (CPMV) and its variants were selected as specific templates due to their high symmetry, scalability, and stability, which have proven useful in materials science applications. While the methods described herein were optimized for the CPMV capsids, they also provide a useful starting point for researchers who are working toward the nanoassembly of metal nanoparticles on other protein scaffolds.
金属纳米级三维结构可将电磁能集中在精确的空间位置，以实现传感和光催化应用。我们已经开发出基于溶液的方法，可重复性地制造用作高效表面增强拉曼光谱（SERS）生物传感器的三维金纳米结构。病毒衣壳被用作模板，将金纳米颗粒组装在其表面的明确位置，以制备纳米级三维结构。豇豆花叶病毒（CPMV）及其变体因其高度对称性、可扩展性和稳定性而被选作特定模板，这些特性已在材料科学应用中得到验证。虽然本文所述方法是针对CPMV衣壳进行优化的，但它们也为致力于在其他蛋白质支架上进行金属纳米颗粒纳米组装的研究人员提供了一个有用的起点。
