Department of Civil, Architectural, and Environmental Engineering, University of Miami, 1251 Memorial Dr. McArthur Engineering Building, Coral Gables, FL 33146-0630, USA.
Department of Civil, Architectural, and Environmental Engineering, University of Miami, 1251 Memorial Dr. McArthur Engineering Building, Coral Gables, FL 33146-0630, USA.
Chemosphere. 2018 Oct;208:196-206. doi: 10.1016/j.chemosphere.2018.05.167. Epub 2018 May 28.
Organic or inorganic stabilizers are often used for coating nanoparticles (NPs) in consumer products. However, upon release of stabilized NPs into the environment, uncertainty exists as to the antimicrobial properties of NPs due to stabilizers and the resultant bioaccumulation in organisms. This study investigates antibacterial effects and subsequent mechanisms of TiO NPs on Escherichia coli (E. coli) in the presence and absence of stabilizers (CMC, PVP, and SiO) commonly used in consumer products. Compared with uncoated TiO NPs, the presence of any stabilizers tested in this study increased toxicity of NPs and enhanced growth inhibition in E. coli. While the particle sizes of TiO were smaller as the result of coating with PVP or CMC and appeared to contribute to E. coli cell damage, the generation of reactive oxygen species (ROS) was independent of stabilizer type. In fact, coating with PVP and CMC exerted ROS scavenging properties. In contrast, increased ROS production was observed at higher concentrations of TiO and upon coating with SiO. This impact of SiO can be related to the formation of a TiOSi chemical bond. The results of the present study emphasize the importance of nanoparticle coating to their anti-bacterial activity and toxicity.
有机或无机稳定剂通常用于涂覆消费品中的纳米颗粒 (NPs)。然而，由于稳定剂的存在以及在生物体中的生物累积，当稳定的 NPs 释放到环境中时，NPs 的抗菌性能存在不确定性。本研究调查了在存在和不存在消费品中常用稳定剂 (CMC、PVP 和 SiO) 的情况下，TiO NPs 对大肠杆菌 (E. coli) 的抗菌作用和后续机制。与未涂覆的 TiO NPs 相比，本研究中测试的任何稳定剂的存在都增加了 NPs 的毒性，并增强了 E. coli 的生长抑制。虽然 PVP 或 CMC 涂覆导致 TiO 的粒径变小，似乎导致大肠杆菌细胞损伤，但活性氧 (ROS) 的产生与稳定剂类型无关。事实上，PVP 和 CMC 的涂覆具有清除 ROS 的特性。相比之下，在较高浓度的 TiO 和涂覆 SiO 时，观察到 ROS 产生增加。这种 SiO 的影响可以与 TiOSi 化学键的形成有关。本研究的结果强调了纳米颗粒涂层对其抗菌活性和毒性的重要性。
