无载药阳离子聚（D,L-丙交酯-乙交酯）纳米粒通过纳米细菌相互作用的抗生物膜效应。
Antibiofilm effect of drug-free and cationic poly(D,L-lactide-co-glycolide) nanoparticles via nano-bacteria interactions.
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Department of Pharmacy, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Nanomedicine (Lond). 2018 May;13(10):1093-1106. doi: 10.2217/nnm-2017-0391. Epub 2018 Jun 6.
AIM
Recently, nano-bio interactions and their biomedical impacts have drawn much attention, but nano-bacteria interaction and its function are unknown. Herein, we aim to synthesize drug-free and cationic nanoparticles (CNPs) and investigate CNP-bacteria interaction and its antibiofilm effect.
MATERIALS & METHODS: The bioactivity of CNPs against Streptococcus mutans was examined by colony-forming units counting and scanning electron microscopy. CNP-bacteria interaction force was measured by atomic force microscopy.
RESULTS
CNPs (217.7 nm, 14.7 mv) showed a concentration-dependent activity against bacteria. Particularly, CNPs at 200 μg/ml completely inhibited planktonic bacterial growth and biofilm formation, and disrupted ∼70% mature biofilm. CNP-bacteria interaction force was up to 184 nN.
CONCLUSION
CNPs have great potentials for convenient local use for prevention and treatment of bacteria-related oral diseases.
目的
近年来，纳米-生物相互作用及其生物医学影响引起了广泛关注，但纳米-细菌相互作用及其功能尚不清楚。本研究旨在合成无药物和阳离子纳米颗粒（CNPs），并研究 CNPs 与细菌的相互作用及其抗生物膜效应。
材料与方法
通过菌落形成单位计数和扫描电子显微镜检查 CNPs 对变形链球菌的生物活性。通过原子力显微镜测量 CNPs-细菌相互作用力。
结果
CNPs（217.7nm，14.7mv）对细菌表现出浓度依赖性活性。特别是，CNPs 在 200μg/ml 时完全抑制浮游细菌的生长和生物膜的形成，并破坏约 70%成熟的生物膜。CNPs-细菌相互作用力高达 184nN。
结论
CNPs 具有方便局部用于预防和治疗与细菌相关的口腔疾病的巨大潜力。
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