Rasmussen Bo S, Sørensen Celine L, Vester-Glowinski Peter V, Herly Mikkel, Kurbegovic Sorel, Ørholt Mathias, Svalgaard Jesper D, Kølle Stig-Frederik T, Kristensen Annemarie T, Talman Maj-Lis M, Drzewiecki Krzysztof T, Fischer-Nielsen Anne
Department of Plastic Surgery, Breast Surgery and Burns, University Hospital of Copenhagen, Copenhagen, Denmark.
Department of Radiology, Stanford University, Stanford, Calif.
Plast Reconstr Surg Glob Open. 2018 Apr 4;6(4):e1735. doi: 10.1097/GOX.0000000000001735. eCollection 2018 Apr.
Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting.
Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days. The stromal vascular fraction (SVF) was isolated from excised fat and liposuctioned fat from different anatomical sites and analyzed. Porcine adipose-derived stem/stromal cells (ASCs) were cultured in different growth supplements, and population doubling time, maximum cell yield, expression of surface markers, and differentiation potential were investigated.
Structural fat grafting in the breast and subcutaneous bolus grafting in the abdomen revealed average graft retention of 53.55% and 15.28%, respectively, which are similar to human reports. Liposuction yielded fewer SVF cells than fat excision, and abdominal fat had the most SVF cells/g fat with SVF yields similar to humans. Additionally, we demonstrated that porcine ASCs can be readily isolated and expanded in culture in allogeneic porcine platelet lysate and fetal bovine serum and that the use of 10% porcine platelet lysate or 20% fetal bovine serum resulted in population doubling time, maximum cell yield, surface marker profile, and trilineage differentiation that were comparable with humans.
The Göttingen minipig is a feasible and cost-effective, large animal model for future translational studies of cell-enriched fat grafting.
富含细胞的脂肪移植在提高移植存活率方面已显示出有前景的结果，尽管许多问题仍未得到解答。大型动物模型对于弥合啮齿动物研究与人体试验之间的差距至关重要。我们提出了一种逐步方法，将哥廷根小型猪用作未来富含细胞的大容量脂肪移植研究的模型。
脂肪移植采用团注注射和结构性脂肪移植。120天后通过磁共振成像评估移植保留情况。从不同解剖部位切除的脂肪和吸脂脂肪中分离出基质血管成分（SVF）并进行分析。将猪脂肪来源的干细胞（ASC）在不同生长补充剂中培养，并研究群体倍增时间、最大细胞产量、表面标志物表达和分化潜能。
乳房的结构性脂肪移植和腹部的皮下团注移植分别显示平均移植保留率为53.55%和15.28%，这与人类报告相似。吸脂获得的SVF细胞比脂肪切除少，腹部脂肪的SVF细胞/克脂肪最多，SVF产量与人类相似。此外，我们证明猪ASC可以很容易地在同种异体猪血小板裂解物和胎牛血清中分离并在培养中扩增，并且使用10%猪血小板裂解物或20%胎牛血清导致的群体倍增时间、最大细胞产量、表面标志物谱和三系分化与人类相当。
哥廷根小型猪是未来富含细胞的脂肪移植转化研究的一种可行且具有成本效益的大型动物模型。
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