由“单端转座”产生的连接序列。
Junction sequences generated by 'one-ended transposition'.
作者信息
Mötsch S, Schmitt R, Avila P, de la Cruz F, Ward E, Grinsted J
出版信息
Nucleic Acids Res. 1985 May 10;13(9):3335-42. doi: 10.1093/nar/13.9.3335.
Abstract
In the presence of the cognate transposase, plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids ('one-ended transposition'). The junctions across the sequences of donor and recipient DNA in recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (it is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tn1721 themselves, suggesting that the mechanism of one-ended transposition is very similar to that of the corresponding entire elements.
摘要
在同源转座酶存在的情况下，含有Tn21或Tn1721单一反向重复（IR）序列的质粒可与其他质粒高效融合（“单端转座”）。已确定了通过此过程产生的重组体中供体和受体DNA序列间的连接。这些结果表明，供体DNA片段精确地起始于IR序列（另一端是可变的），并由宿主DNA的正向重复序列（通常为5bp）侧翼包围，该正向重复序列在原始宿主序列中仅出现一次。这些是Tn21和Tn1721自身转座产生的重组体的特征，表明单端转座机制与相应完整元件的转座机制非常相似。
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