关于转座的分子机制
On the molecular mechanisms of transposition.
作者信息
Galas D J, Chandler M
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4858-62. doi: 10.1073/pnas.78.8.4858.
Abstract
We present a model for transposition that allows a choice between cointegrate formation (replicon fusion) and direct transposition. We propose that initiation of the process occurs by invasion of the target DNA by a single-stranded end of the transposable element. This leads to nicking of one of the DNA strands of the target molecule and ligation of this strand to that of the invading transposon. Transposition then occurs in a processive way by replication of the element from the invading end into the target site in a looped rolling-circle mode similar to replication of phage phi X174 replicative form to viral strand. The choice between cointegrate formation and direct transposition occurs at the nick-ligation step, which terminates the process. We suggest that the choice is determined by the topology of the transposition enzymes and could be related to whether the element generates five- or nine-base-pair repeats in the target DNA on insertion.
摘要
我们提出了一种转座模型，该模型允许在共整合体形成（复制子融合）和直接转座之间进行选择。我们认为，该过程的起始是由转座元件的单链末端侵入靶DNA引发的。这会导致靶分子的一条DNA链产生切口，并将该链与侵入的转座子的链连接起来。然后，转座以连续的方式进行，通过从侵入末端开始将元件复制到靶位点，以环状滚环模式进行，类似于噬菌体phi X174复制型到病毒链的复制。共整合体形成和直接转座之间的选择发生在切口连接步骤，该步骤终止了该过程。我们认为，这种选择由转座酶的拓扑结构决定，并且可能与元件插入时在靶DNA中产生五碱基对或九碱基对重复序列有关。
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