Pifer M L, Smith H O
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3731-5. doi: 10.1073/pnas.82.11.3731.
A plasmid system was used to investigate the processing of donor DNA during transformation of competent Haemophilus influenzae. Using biochemical and genetic methods, we have determined that portion of a donor plasmid molecule that, on average, can become integrated into a homologous recipient plasmid during transformation. Our results show that (i) transformation efficiency decreases linearly with donor DNA length over the range of 11 to 3.5 kilobase pairs, (ii) transformation efficiency decreases exponentially with size for donor molecules less than 3.5 kilobase pairs in length, and (iii) 5'-end label, but not 3'-end label, can be specifically incorporated into the resident homologous region. We present a model for donor processing during entry that encompasses and explains these observations.
一种质粒系统被用于研究感受态流感嗜血杆菌转化过程中供体DNA的加工情况。通过生化和遗传学方法，我们已经确定了供体质粒分子的一部分，平均而言，在转化过程中它能够整合到同源受体质粒中。我们的结果表明：（i）在11至3.5千碱基对的范围内，转化效率随供体DNA长度呈线性下降；（ii）对于长度小于3.5千碱基对的供体分子，转化效率随大小呈指数下降；（iii）5'-末端标记而非3'-末端标记能够特异性地整合到常驻同源区域。我们提出了一个关于供体进入过程中加工的模型，该模型涵盖并解释了这些观察结果。
