质粒在感受态流感嗜血杆菌中的建立是通过非同源转化发生的。
Plasmid establishment in competent Haemophilus influenzae occurs by illegitimate transformation.
作者信息
Pifer M L
出版信息
J Bacteriol. 1986 Nov;168(2):683-7. doi: 10.1128/jb.168.2.683-687.1986.
Abstract
Establishment of plasmids in naturally competent Haemophilus influenzae is incompatible with transformation via the normal DNA translocation pathways. Instead, establishing plasmids appear to evade the degradation which ordinarily accompanies translocation, arriving as intact double-stranded molecules in the cytoplasm. Evidence is presented that plasmid establishment is a rare illegitimate transformation event which resembles artificial transformation. This process is compared with plasmid marker rescue transformation, and a method for greatly increasing establishment frequency is described.
摘要
在天然感受态流感嗜血杆菌中建立质粒与通过正常DNA转位途径进行的转化不兼容。相反，建立质粒似乎能规避通常伴随转位的降解，以完整的双链分子形式进入细胞质。有证据表明，质粒建立是一种罕见的非法转化事件，类似于人工转化。将此过程与质粒标记拯救转化进行了比较，并描述了一种大幅提高建立频率的方法。
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本文引用的文献
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Genetic transformation.遗传转化
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Transformation of Haemophilus influenzae by plasmid RSF0885.质粒RSF0885介导的流感嗜血杆菌转化
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