Department of Biological Sciences, University of Toledo, 2801W. Bancroft, Toledo, OH, 43607, USA.
Cell Biology Program, The Hospital for Sick Children, Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Nat Commun. 2018 Jun 7;9(1):2210. doi: 10.1038/s41467-018-04678-8.
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that the sperm centrosome contains, in addition to the known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical centriole (distal centriole, DC) composed of splayed microtubules surrounding previously undescribed rods of centriole luminal proteins. The sperm centrosome is remodeled by both reduction and enrichment of specific proteins and the formation of these rods during spermatogenesis. In vivo and in vitro investigations show that the flagellum-attached, atypical DC is capable of recruiting PCM, forming a daughter centriole, and localizing to the spindle pole during mitosis. Altogether, we show that the DC is compositionally and structurally remodeled into an atypical centriole, which functions as the zygote's second centriole. These findings now provide novel avenues for diagnostics and therapeutic strategies for male infertility, and insights into early embryo developmental defects.
人类受精过程中中心体的遗传仍然神秘。在这里，我们发现精子中心体除了已知的典型桶状中心粒（近端中心粒，PC）外，还包含一个周围基质（中心粒周围物质，PCM）和一个由以前未描述的中心粒腔蛋白棒组成的非典型中心粒（远端中心粒，DC），由展开的微管围绕。精子中心体在精子发生过程中通过特定蛋白质的减少和富集以及这些棒的形成进行重塑。体内和体外研究表明，与鞭毛相连的非典型 DC 能够募集 PCM，形成一个子中心粒，并在有丝分裂过程中定位到纺锤体极。总的来说，我们表明 DC 在组成和结构上被重塑为一个非典型的中心粒，它作为受精卵的第二个中心粒发挥作用。这些发现为男性不育症的诊断和治疗策略提供了新的途径，并深入了解早期胚胎发育缺陷。
