Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, Bejaia, Algeria.
Department of Microbiology, National Center of Public Health Laboratories (NCPHL), Sanaa, Yemen.
Microb Drug Resist. 2018 Dec;24(10):1537-1542. doi: 10.1089/mdr.2018.0024. Epub 2018 Jun 8.
The aim of this study was to characterize the O25b/ST131 clone in ciprofloxacin-resistant isolates from Yemen. A total of 41 ciprofloxacin-resistant strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes and . Genetic relatedness of isolates was determined by pulsed-field gel electrophoresis (PFGE). O25b/ST131 clone detection was performed using polymerase chain reaction of O25b and allele 3 of the gene and by a multilocus sequence typing. All isolates contained the 'r gene associated with and genes in 63.4% and 12.2%, respectively. A rate of 36.6% (15/41) of O25b/ST131 isolates were identified belonging to the H30-Rx subclone producing both CTX-M-15 and Aac(6')Ib-cr enzymes and carrying two substitutions in GyrA (Ser83Leu/Asp87Asn) and two substitutions in ParC (Ser80Ile/Glu84Val). Most of them were uropathogenic unrelated isolates recovered from outpatients. This is the first report of a high prevalence of O25b/ST131 from Yemen.
本研究旨在对也门耐环丙沙星的 分离株中 O25b/ST131 克隆进行特征描述。2013 年 1 月至 12 月，从也门萨那的住院和门诊患者的临床标本中收集了 41 株耐环丙沙星的 菌株。采用聚合酶链反应扩增和测序法检测质粒介导的喹诺酮耐药决定因子、扩展型β-内酰胺酶基因和靶基因 和 中喹诺酮耐药决定区的突变。采用脉冲场凝胶电泳（PFGE）确定分离株的遗传相关性。通过聚合酶链反应检测 O25b 和 基因的等位基因 3 对 O25b/ST131 克隆进行检测，并进行多位点序列分型。所有分离株均携带与 和 基因相关的 r 基因，分别占 63.4%和 12.2%。36.6%（15/41）的 O25b/ST131 分离株属于 H30-Rx 亚克隆，该亚克隆可同时产生 CTX-M-15 和 Aac(6')Ib-cr 酶，并在 GyrA（Ser83Leu/Asp87Asn）和 ParC（Ser80Ile/Glu84Val）中有两个取代。其中大多数为从门诊患者中分离出的无关联的尿路致病性 分离株。这是首次报道也门 O25b/ST131 高流行率。
