Mleczko Anna M, Celichowski Piotr, Bąkowska-Żywicka Kamilla
Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego St. 12/14, 61-704 Poznan, Poland.
Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego St. 12/14, 61-704 Poznan, Poland.
Biochim Biophys Acta Gene Regul Mech. 2018 Jun 6. doi: 10.1016/j.bbagrm.2018.06.001.
Ribosome-associated noncoding (ranc) RNAs are a novel class of short regulatory RNAs with functions and origins that have not been well studied. In this present study, we functionally characterized the molecular activity of Saccharomyces cerevisiae transfer RNA (tRNA)-derived fragments (tRFs) during protein biosynthesis. Our results indicate ribosome-associated tRFs derived from both 5' (ranc-5'-tRFs) and 3'-part of tRNAs (ranc-3'-tRFs) have regulatory roles during translation. We demonstrated five 3'-tRFs and one 5'-tRF associate with a small ribosomal subunit and aminoacyl-tRNA synthetases (aa-RSs) in yeast. Furthermore, we discovered that four yeast aa-RSs interact directly with yeast ribosomes. tRFs interactions with ribosome-associated aa-RSs correlate with impaired efficiency of tRNA aminoacylation.
核糖体相关非编码（ranc）RNA是一类新型的短调节RNA，其功能和起源尚未得到充分研究。在本研究中，我们对酿酒酵母转移RNA（tRNA）衍生片段（tRFs）在蛋白质生物合成过程中的分子活性进行了功能表征。我们的结果表明，来自tRNA 5'端（ranc-5'-tRFs）和3'端（ranc-3'-tRFs）的核糖体相关tRFs在翻译过程中具有调节作用。我们证明了5个3'-tRFs和1个5'-tRF与酵母中的小核糖体亚基和氨酰-tRNA合成酶（aa-RSs）相关联。此外，我们发现4种酵母aa-RSs与酵母核糖体直接相互作用。tRFs与核糖体相关aa-RSs的相互作用与tRNA氨酰化效率受损相关。
