牛呼吸道合胞体病毒感染增强了多杀性巴氏杆菌对呼吸道上皮细胞的黏附。
Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells.
机构信息
Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
出版信息
Vet Microbiol. 2018 Jul;220:33-38. doi: 10.1016/j.vetmic.2018.04.031. Epub 2018 Apr 30.
Primary infection with bovine respiratory syncytial virus (BRSV) predisposes cattle to secondary infection with bacteria that cause bovine respiratory disease complex (BRDC). However, the interaction between BRSV and bacteria is unclear. This in vitro study examined the adherence of Pasteurella multocida (PM) to BRSV-infected cells was assessed in colony forming unit assays, by flow cytometry analysis, and by indirect immunofluorescence analysis (IFA) of epithelial cells (A549, HEp-2, and MDBK). An in vitro model based on infection of BRSV-infected epithelial cells revealed that PM adherence to BRSV-infected cells was 2- to 8-fold higher than uninfected cells. This was confirmed by flow cytometry analysis and IFA. Epithelial cell expression of mRNA encoding cytokines and chemokines increased after exposure to PM, but increased further after co-infection with BRSV and PM. BRSV-mediated adherence of PM to epithelial cells may underlie the serious symptoms of BRDC.
牛呼吸道合胞体病毒（BRSV）的初次感染使牛易受到引起牛呼吸道疾病复合症（BRDC）的细菌的二次感染。然而，BRSV 和细菌之间的相互作用尚不清楚。本体外研究通过集落形成单位测定、流式细胞术分析和上皮细胞（A549、HEp-2 和 MDBK）的间接免疫荧光分析（IFA），评估了多杀巴斯德氏菌（PM）对 BRSV 感染细胞的黏附作用。基于 BRSV 感染上皮细胞的体外模型显示，PM 对 BRSV 感染细胞的黏附作用比未感染细胞高 2-8 倍。这通过流式细胞术分析和 IFA 得到了证实。上皮细胞暴露于 PM 后细胞因子和趋化因子编码 mRNA 的表达增加，而与 BRSV 和 PM 共同感染后则进一步增加。BRSV 介导的 PM 对上皮细胞的黏附可能是 BRDC 严重症状的基础。
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