IRRIV-International Renal Research Institute Vicenza, Vicenza, Italy.
Department of Internal Medicine and Nephrology, King Abdulaziz University Hospital, Jeddah, Saudi Arabia.
Blood Purif. 2018;46(3):187-195. doi: 10.1159/000489921. Epub 2018 Jun 8.
BACKGROUND/AIMS: The use of adsorption cartridges for hemoperfusion (HP) is rapidly evolving. For these devices, the potential induced cytotoxicity is an important issue. The aim of this study was to investigate potential in vitro cytotoxic effects of different sorbent cartridges, HA130, HA230, HA330, HA380 (Jafron, China), on U937 monocytes.
Monocytes were exposed to the sorbent material in static and dynamic manners. In static test, cell medium samples were collected after 24 h of incubation in the cartridges. In dynamic test, HP modality has been carried out and samples at 30, 60, 90, and 120 min were collected.
Compared to control samples, there was no evidence of increased necrosis or apoptosis in monocytes exposed to the cartridges both in the static and dynamic tests.
Our in vitro testing suggests that HA cartridges carry an optimal level of biocompatibility and their use in HP is not associated with adverse reactions or signs of cytotoxicity.
背景/目的：血液灌流（HP）中吸附柱的使用正在迅速发展。对于这些设备，潜在的诱导细胞毒性是一个重要问题。本研究的目的是研究不同吸附剂柱，HA130、HA230、HA330、HA380（中国捷扶康）对 U937 单核细胞的体外潜在细胞毒性作用。
以静态和动态方式将单核细胞暴露于吸附材料。在静态试验中，将吸附柱孵育 24 小时后收集细胞培养基样本。在动态试验中，进行 HP 模式，并在 30、60、90 和 120 分钟时收集样本。
与对照样本相比，无论是在静态还是动态试验中，暴露于吸附柱的单核细胞均未显示出坏死或凋亡增加的证据。
我们的体外测试表明，HA 柱具有最佳的生物相容性，其在 HP 中的使用与不良反应或细胞毒性迹象无关。
