发光共轭低聚噻吩探针在组织中纯淀粉样纤维和蛋白质聚集体荧光成像的应用
Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues.
作者信息
Nilsson K Peter R, Lindgren Mikael, Hammarström Per
机构信息
IFM-Department of Chemistry, Linköping University, Linköping, Sweden.
Department of Physics, The Norwegian University of Science and Technology, 7491, Trondheim, Norway.
出版信息
Methods Mol Biol. 2018;1779:485-496. doi: 10.1007/978-1-4939-7816-8_30.
Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we outline updated protocols for the application of the anionic pentameric LCO, p-FTAA, for staining and hyperspectral imaging of protein aggregates in a variety of settings such as in vitro formed amyloid fibrils, ex vivo tissue sections, and whole brain Drosophila.
发光共轭寡聚噻吩和聚噻吩（LCOs和LCPs）是用于对与淀粉样变性相关的大量蛋白质聚集体进行光学成像的宝贵工具。在此，我们概述了应用阴离子五聚体LCO（p-FTAA）在多种环境下对蛋白质聚集体进行染色和高光谱成像的更新方案，这些环境包括体外形成的淀粉样原纤维、离体组织切片和全脑果蝇。
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