Brodie C, Bak A, Sampson S R
Brain Res. 1985 Jun 17;336(2):384-6. doi: 10.1016/0006-8993(85)90674-2.
This study was undertaken in order to determine the relation among cell fusion, [3H]ouabain binding and the membrane potential (Em) of cultured rat skeletal muscle. The amount of ouabain bound and the Em both increased with age, the increases being most dramatic following fusion. Inhibition of fusion prevented the developmental increases in both properties of cultured muscle. After fusion, the size of the electrogenic component of Em, determined by the decrease in Em produced by ouabain within 5-10 min, increased independent of the age at which fusion occurred. It is concluded that the increase in Em with age depends on postfusion appearance and activity of Na,K-ATPase.
进行本研究是为了确定培养的大鼠骨骼肌细胞融合、[3H]哇巴因结合与膜电位（Em）之间的关系。哇巴因结合量和Em均随年龄增加，融合后增加最为显著。融合抑制可阻止培养肌肉这两种特性的发育性增加。融合后，通过哇巴因在5 - 10分钟内使Em降低所确定的Em的电生成成分大小增加，且与融合发生时的年龄无关。结论是，Em随年龄增加取决于融合后钠钾ATP酶的出现和活性。
