Nigg E A, Schäfer G, Hilz H, Eppenberger H M
Cell. 1985 Jul;41(3):1039-51. doi: 10.1016/s0092-8674(85)80084-2.
The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison of the R II distribution with the location of a bona fide Golgi membrane constituent, this area was identified as the Golgi complex. The cytochemical localization of R II was confirmed by subcellular fractionation. In addition, cAMP-dPK II was associated with microtubule-organizing centers, in particular with mitotic spindle poles. These distributions of cAMP-dPK II probably represent important factors in mediating the effects of cAMP on basic cellular activities ranging from secretion and proliferation to cell shape and motility.
通过间接免疫荧光显微镜法确定了环磷酸腺苷依赖性蛋白激酶II型酶（cAMP - dPK II）在上皮细胞和成纤维细胞中的亚细胞分布。在间期细胞中，调节亚基（R II）和催化亚基（C）都集中在核周区域。通过将R II的分布与真正的高尔基体膜成分的位置进行比较，该区域被确定为高尔基体复合体。R II的细胞化学定位通过亚细胞分级分离得到证实。此外，cAMP - dPK II与微管组织中心相关，特别是与有丝分裂纺锤体极相关。cAMP - dPK II的这些分布可能是介导cAMP对从分泌、增殖到细胞形状和运动等基本细胞活动产生影响的重要因素。
