Keresztes M, Takács O, Guba F
Cell Differ. 1985 Apr;16(2):133-7. doi: 10.1016/0045-6039(85)90526-3.
Chick embryos were treated with 4-aminopyridine (4 X 100 micrograms) during a critical stage of muscle development, and the effect of enhanced neuromuscular activity upon energy metabolism was studied in two fast-twitch muscles and a slow-tonic muscle. In the slow-tonic muscles of treated embryos, the specific activities of creatine kinase (CK) and lactate dehydrogenase (LDH) were reduced by 11 and 21%, respectively, compared with control values, whereas the ratios of the CK-MB isoforms and the LDH-H subunits increased to 125 and 135% of the control values, respectively. No significant changes could be shown in the enzymatic pattern of fast muscles. These results indicate that a moderate increase in neuromuscular activity of the chick embryo primarily influences the metabolism of developing slow muscles, promoting the development of an enzyme profile characteristic of slow oxidative fibres.
在肌肉发育的关键阶段，用4-氨基吡啶（4×100微克）处理鸡胚，并在两块快肌和一块慢张力肌中研究增强的神经肌肉活动对能量代谢的影响。与对照值相比，处理过的胚胎的慢张力肌中肌酸激酶（CK）和乳酸脱氢酶（LDH）的比活性分别降低了11%和21%，而CK-MB同工型和LDH-H亚基的比例分别增加到对照值的125%和135%。快肌的酶谱没有显著变化。这些结果表明，鸡胚神经肌肉活动的适度增加主要影响发育中的慢肌代谢，促进具有慢氧化纤维特征的酶谱的发育。
