Simon D, Searls D B, Cao Y, Sun K, Knowles B B
Cytogenet Cell Genet. 1985;39(2):116-20. doi: 10.1159/000132118.
The single site of integration of hepatitis B virus in the human hepatocellular carcinoma cell line Hep 3B 2-1/7 was found to segregate with human chromosome 12 in somatic cell hybrids. Analysis of metaphase spreads of Hep 3B 2-1/7 following in situ hybridization with pHBV revealed integration at 12q13----q14, a location that coincides with a fragile site, fra (12q13). The possible significance of this location to the development of hepatocellular carcinomas is discussed.
在人肝癌细胞系Hep 3B 2-1/7中，乙肝病毒的单一整合位点在体细胞杂种中与人类12号染色体分离。用pHBV进行原位杂交后，对Hep 3B 2-1/7中期染色体铺展进行分析，发现整合位点位于12q13----q14，此位置与一个脆性位点fra (12q13) 重合。文中讨论了该位置对肝癌发生的可能意义。
