Simon D, Knowles B B
Lab Invest. 1986 Dec;55(6):657-65.
A cell line derived from the biopsy of a human hepatocellular carcinoma which retains the differentiated phenotype of the liver parenchymal cell is described. Comparison of the integration sites of hepatitis B virus within the cellular genome of the biopsy specimen and within the genome of the multiply-passaged cell line reveals five stable sites of viral integration in the host cell genome. Multiple chromosome abnormalities are found in this cell line, some in the same area of the genome in which abnormalities were found in other human hepatomas. Chromosomes obtained from the peripheral blood cultures of the same patient manifest multiple signs of chromosome instability including breaks, minutes, chromosome pulverization, and acentric fragments, some of which localize to the chromosomal sites involved in the abnormalities in the tumor cell line. Chromosomal instability and/or virus-induced chromosome damage as factors in the etiology of human hepatocellular carcinoma are discussed.
本文描述了一种源自人肝细胞癌活检组织的细胞系，该细胞系保留了肝实质细胞的分化表型。对活检标本细胞基因组内和多次传代细胞系基因组内乙型肝炎病毒整合位点的比较显示，宿主细胞基因组中有五个稳定的病毒整合位点。在该细胞系中发现了多种染色体异常，其中一些位于与其他人类肝癌中发现异常的相同基因组区域。从同一患者外周血培养物中获得的染色体表现出多种染色体不稳定迹象，包括断裂、微小体、染色体粉碎和无着丝粒片段，其中一些定位于肿瘤细胞系异常所涉及的染色体位点。文中讨论了染色体不稳定和/或病毒诱导的染色体损伤作为人类肝细胞癌病因学因素的情况。
