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人类主要组织相容性复合体的SB亚区：基因组织、等位基因多态性及在转化细胞中的表达

SB subregion of the human major histocompatibility complex: gene organization, allelic polymorphism and expression in transformed cells.

作者信息

Okada K, Prentice H L, Boss J M, Levy D J, Kappes D, Spies T, Raghupathy R, Mengler R A, Auffray C, Strominger J L

出版信息

EMBO J. 1985 Mar;4(3):739-48. doi: 10.1002/j.1460-2075.1985.tb03691.x.

DOI:10.1002/j.1460-2075.1985.tb03691.x
PMID:2988934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554250/
Abstract

The SB region of the human major histocompatibility complex (MHC) has been cloned from cosmid and lambda phage libraries made from the human B-lymphoblastoid cell line Priess (DR4/4, DC4/4, SB3/4). Two alpha genes and two beta genes are encoded in the 100 kb long SB region in the order SB alpha-SB beta-SX alpha-SX beta. The SB alpha and SB beta genes encode the alpha and beta subunits of the SB subset of class II MHC molecules. Both the SX alpha and the SX beta genes are pseudogenes in the haplotype examined. From the isolated clones, the two haplotypes of the Priess cell line, SB3 and SB4, are distinguished by nucleotide sequencing and blot hybridization analyses. Restriction site polymorphisms between the SB3 and SB4 clones were observed only in relatively small regions of the SB beta and SX beta genes. A mouse macrophage cell line was transfected with one of the cosmid clones containing both SB alpha and SB beta genes. Expression of the alpha and beta genes was detected by fluorescene-activated cell sorting (FACS) and two-dimensional gel electrophoresis using SB-specific monoclonal antibodies.

摘要

人类主要组织相容性复合体（MHC）的SB区域已从由人类B淋巴母细胞系Priess（DR4/4、DC4/4、SB3/4）构建的黏粒和λ噬菌体文库中克隆出来。在长达100 kb的SB区域中，按SBα-SBβ-SXα-SXβ的顺序编码两个α基因和两个β基因。SBα和SBβ基因编码II类MHC分子SB亚群的α和β亚基。在所检测的单倍型中，SXα和SXβ基因均为假基因。通过核苷酸测序和印迹杂交分析，从分离的克隆中区分出Priess细胞系的两种单倍型SB3和SB4。仅在SBβ和SXβ基因的相对较小区域观察到SB3和SB4克隆之间的限制性位点多态性。用其中一个包含SBα和SBβ基因的黏粒克隆转染小鼠巨噬细胞系。使用SB特异性单克隆抗体，通过荧光激活细胞分选（FACS）和二维凝胶电泳检测α和β基因的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/06786a44844a/emboj00268-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/ab9f71a7d8bc/emboj00268-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/dd736d9e54aa/emboj00268-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/baafe5920177/emboj00268-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/2f8875a2c97d/emboj00268-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/06786a44844a/emboj00268-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/ab9f71a7d8bc/emboj00268-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/dd736d9e54aa/emboj00268-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/baafe5920177/emboj00268-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/2f8875a2c97d/emboj00268-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6015/554250/06786a44844a/emboj00268-0168-a.jpg

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本文引用的文献

1
Polymorphism and complexity of the human DC and murine I-A alpha chain genes.人类DC和小鼠I-Aα链基因的多态性与复杂性
EMBO J. 1983;2(1):121-4. doi: 10.1002/j.1460-2075.1983.tb01392.x.
2
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Gene. 1980 Jun;10(1):63-7. doi: 10.1016/0378-1119(80)90144-4.
3
SB: a new HLA-linked human histocompatibility gene defined using HLA-mutant cell lines.SB：一种利用HLA突变细胞系定义的新的与HLA连锁的人类组织相容性基因。
Immunogenetics. 1995;41(1):29-37. doi: 10.1007/BF00188429.
4
Linkage map of three HLA-DR beta-chain genes: evidence for a recent duplication event.三个HLA - DRβ链基因的连锁图谱：近期发生基因复制事件的证据。
Proc Natl Acad Sci U S A. 1985 Nov;82(21):7197-201. doi: 10.1073/pnas.82.21.7197.
5
Sequence analysis of the human major histocompatibility gene SX alpha.人类主要组织相容性基因SXα的序列分析
Mol Cell Biol. 1985 Oct;5(10):2677-83. doi: 10.1128/mcb.5.10.2677-2683.1985.
6
Structural organization of the DR subregion of the human major histocompatibility complex.人类主要组织相容性复合体DR亚区的结构组织
Proc Natl Acad Sci U S A. 1985 Aug;82(15):5165-9. doi: 10.1073/pnas.82.15.5165.
7
Structure and expression of HLA-DQ alpha and -DX alpha genes: interallelic alternate splicing of the HLA-DQ alpha gene and functional splicing of the HLA-DQ alpha gene using a retroviral vector.HLA-DQα和-DXα基因的结构与表达：HLA-DQα基因的等位基因间可变剪接以及利用逆转录病毒载体对HLA-DQα基因进行功能性剪接
Immunogenetics. 1987;26(1-2):63-73. doi: 10.1007/BF00345456.
8
Immunochemistry of the HLA class II molecules isolated from a mouse cell transfected with DQ alpha and beta genes from a DR4 haplotype.从转染了DR4单倍型的DQα和β基因的小鼠细胞中分离出的HLA II类分子的免疫化学。
Immunogenetics. 1987;26(1-2):40-7. doi: 10.1007/BF00345453.
9
Biochemical polymorphism of the HLA-DP heavy chain.HLA - DP重链的生化多态性
Immunogenetics. 1987;25(6):403-7. doi: 10.1007/BF00396107.
10
A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer.鸡主要组织相容性复合体的分子图谱：Ⅱ类β基因与Ⅰ类基因和核仁组织区紧密连锁。
EMBO J. 1988 Sep;7(9):2775-85. doi: 10.1002/j.1460-2075.1988.tb03132.x.
Nature. 1981 Oct 29;293(5835):747-9. doi: 10.1038/293747a0.
4
Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO.家系研究在HLA - DR和GLO之间确定了一个新的组织相容性位点SB。
Nature. 1981 Oct 29;293(5835):745-7. doi: 10.1038/293745a0.
5
The amino acid sequence and gene organization of the heavy chain of the HLA-DR antigen: homology to immunoglobulins.HLA - DR抗原重链的氨基酸序列与基因结构：与免疫球蛋白的同源性
Proc Natl Acad Sci U S A. 1982 Oct;79(19):6013-7. doi: 10.1073/pnas.79.19.6013.
6
cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain.人B细胞同种异体抗原DC1重链的cDNA克隆：与HLA - DR重链有高度序列同源性。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6337-41. doi: 10.1073/pnas.79.20.6337.
7
Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer.通过DNA介导的基因转移鉴定小鼠主要组织相容性复合体的I类基因。
Nature. 1982 Nov 18;300(5889):231-7. doi: 10.1038/300231a0.
8
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10
Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.H-2 Ⅱ类基因的基因转移：小鼠成纤维细胞和仓鼠 B 细胞系的抗原呈递
Cell. 1984 Feb;36(2):319-27. doi: 10.1016/0092-8674(84)90225-3.