Auffray C, Ben-Nun A, Roux-Dosseto M, Germain R N, Seidman J G, Strominger J L
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138, USA.
EMBO J. 1983;2(1):121-4. doi: 10.1002/j.1460-2075.1983.tb01392.x.
A cDNA clone encoding the human B cell alloantigen DC alpha chain (pDCH1) has been used to analyse the structure of the human and murine major histocompatibility complexes by the DNA filter hybridization technique. The pDCH1 probe hybridizes to a single DNA sequence present on chromosome 17 in the mouse genome. A restriction enzyme polymorphism enables us to map this sequence to the I-A subregion. Extensive restriction enzyme polymorphism detected in HLA-DR homozygous typing cells is reminiscent of the DR/MT linkage disequilibrium groups, suggesting that the pDCH1 probe could be useful for haplotype typing in the human population. The HLA-DR region appears more complex than the I region since a second DC-like hybridizing sequence is detected in the human genome in these experiments.
一个编码人类B细胞同种异体抗原DCα链（pDCH1）的cDNA克隆已被用于通过DNA滤膜杂交技术分析人类和小鼠主要组织相容性复合体的结构。pDCH1探针与小鼠基因组中17号染色体上存在的单一DNA序列杂交。一种限制性内切酶多态性使我们能够将该序列定位到I-A亚区。在HLA-DR纯合定型细胞中检测到的广泛限制性内切酶多态性让人想起DR/MT连锁不平衡组，这表明pDCH1探针可能有助于人类群体中的单倍型分型。由于在这些实验中在人类基因组中检测到第二个类似DC的杂交序列，HLA-DR区域似乎比I区域更复杂。
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