Magee A I, Courtneidge S A
EMBO J. 1985 May;4(5):1137-44. doi: 10.1002/j.1460-2075.1985.tb03751.x.
Labelling of cultured cells with [3H]palmitic and [3H]myristic acids demonstrates that each of these fatty acids modifies a substantially different subset of cellular proteins. Hydroxylamine treatment can be used to differentiate sensitive thioester linkages to palmitate from insensitive amide linkages to myristate. Several palmitoylated proteins are surface-oriented glycoproteins while all of the myristylated proteins appear to be internal. Myristate addition is much more tightly coupled to protein synthesis than palmitoylation, which is able to continue at a reduced level even in the prolonged absence of protein synthesis. Acyl proteins patterns were affected both qualitatively and quantitatively by transformation and growth status. The preferential addition of palmitate to the transferrin receptor and myristate to pp60src, and the absence of these modifications from several other proteins is reported. We propose a nomenclature for fatty acyl proteins based on these observations.
用[3H]棕榈酸和[3H]肉豆蔻酸对培养细胞进行标记表明，这些脂肪酸中的每一种都修饰了细胞蛋白质中一个截然不同的子集。羟胺处理可用于区分对棕榈酸敏感的硫酯键和对肉豆蔻酸不敏感的酰胺键。几种棕榈酰化蛋白是面向表面的糖蛋白，而所有肉豆蔻酰化蛋白似乎都在细胞内部。与棕榈酰化相比，肉豆蔻酸的添加与蛋白质合成的耦合更为紧密，即使在长时间缺乏蛋白质合成的情况下，棕榈酰化仍能以较低水平继续进行。酰基化蛋白质模式在质量和数量上都受到转化和生长状态的影响。据报道，转铁蛋白受体优先添加棕榈酸，pp60src优先添加肉豆蔻酸，而其他几种蛋白质则没有这些修饰。基于这些观察结果，我们提出了一种脂肪酰基化蛋白质的命名法。
