正十四烷酰基是来自牛心肌的环磷酸腺苷依赖性蛋白激酶催化亚基的氨基末端阻断基团。
n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.
作者信息
Carr S A, Biemann K, Shoji S, Parmelee D C, Titani K
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6128-31. doi: 10.1073/pnas.79.20.6128.
Abstract
The unusual NH2-terminal blocking group of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein was found to be amide-linked n-tetradecanoic acid by gas chromatographic-, direct chemical ionization-, and fast atom bombardment-mass spectrometry. In addition, fast atom bombardment mass spectrometry revealed the presence of an additional alanine which had been overlooked when the original sequence was determined. The corrected and completed NH2-terminal sequence of the 350-amino acid catalytic subunit is CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys.
摘要
通过气相色谱法、直接化学电离法和快原子轰击质谱法发现，牛心肌环磷酸腺苷依赖性蛋白催化亚基异常的氨基末端阻断基团是酰胺连接的正十四烷酸。此外，快原子轰击质谱法显示存在一个额外的丙氨酸，该丙氨酸在确定原始序列时被忽视了。350个氨基酸的催化亚基经校正和完整的氨基末端序列为CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys。
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