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正十四烷酰基是来自牛心肌的环磷酸腺苷依赖性蛋白激酶催化亚基的氨基末端阻断基团。

n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.

作者信息

Carr S A, Biemann K, Shoji S, Parmelee D C, Titani K

出版信息

Proc Natl Acad Sci U S A. 1982 Oct;79(20):6128-31. doi: 10.1073/pnas.79.20.6128.

DOI:10.1073/pnas.79.20.6128
PMID:6959104
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347072/
Abstract

The unusual NH2-terminal blocking group of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein was found to be amide-linked n-tetradecanoic acid by gas chromatographic-, direct chemical ionization-, and fast atom bombardment-mass spectrometry. In addition, fast atom bombardment mass spectrometry revealed the presence of an additional alanine which had been overlooked when the original sequence was determined. The corrected and completed NH2-terminal sequence of the 350-amino acid catalytic subunit is CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys.

摘要

通过气相色谱法、直接化学电离法和快原子轰击质谱法发现，牛心肌环磷酸腺苷依赖性蛋白催化亚基异常的氨基末端阻断基团是酰胺连接的正十四烷酸。此外，快原子轰击质谱法显示存在一个额外的丙氨酸，该丙氨酸在确定原始序列时被忽视了。350个氨基酸的催化亚基经校正和完整的氨基末端序列为CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys。

相似文献

1
n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.正十四烷酰基是来自牛心肌的环磷酸腺苷依赖性蛋白激酶催化亚基的氨基末端阻断基团。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6128-31. doi: 10.1073/pnas.79.20.6128.
2
Identification of the NH2-terminal blocking group of calcineurin B as myristic acid.鉴定钙调神经磷酸酶B的氨基末端封闭基团为肉豆蔻酸。
FEBS Lett. 1982 Dec 27;150(2):314-8. doi: 10.1016/0014-5793(82)80759-x.
3
Identification of the NH2-terminal blocking group of NADH-cytochrome b5 reductase as myristic acid and the complete amino acid sequence of the membrane-binding domain.鉴定NADH-细胞色素b5还原酶的氨基末端封闭基团为肉豆蔻酸以及膜结合结构域的完整氨基酸序列。
J Biol Chem. 1984 Nov 10;259(21):13349-54.
4
Rapid identification of NH2-terminal myristyl peptides by reversed-phase high-performance liquid chromatography.通过反相高效液相色谱法快速鉴定氨基末端肉豆蔻酰化肽段
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本文引用的文献

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Fatty acid acylation of proteins in cultured cells.培养细胞中蛋白质的脂肪酸酰化作用。
J Biol Chem. 1980 Nov 10;255(21):10021-4.
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Fast-atom-bombardment mass spectra of enkephalins.脑啡肽的快原子轰击质谱图。
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Primary structure of the regulatory subunit of type II cAMP-dependent protein kinase from bovine cardiac muscle.来自牛心肌的II型环磷酸腺苷依赖性蛋白激酶调节亚基的一级结构。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2544-8. doi: 10.1073/pnas.79.8.2544.
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Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase.牛心肌环磷酸腺苷依赖性蛋白激酶催化亚基的完整氨基酸序列。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):848-51. doi: 10.1073/pnas.78.2.848.
5
Mutants of vesicular stomatitis virus blocked at different stages in maturation of the viral glycoprotein.水泡性口炎病毒的突变体在病毒糖蛋白成熟的不同阶段受阻。
Cell. 1980 Sep;21(2):417-27. doi: 10.1016/0092-8674(80)90478-x.
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Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane.脂质与蛋白质的共价结合。大肠杆菌外膜胞壁质脂蛋白N端的甘油二酯和酰胺连接的脂肪酸。
Eur J Biochem. 1973 Apr;34(2):284-96. doi: 10.1111/j.1432-1033.1973.tb02757.x.
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Studies on the orientation of cyclic AMP-dependent protein kinase in human erythrocyte membranes.人红细胞膜中环磷酸腺苷依赖性蛋白激酶定位的研究。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3735-8. doi: 10.1073/pnas.70.12.3735.
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Posttranslational covalent modification of proteins.蛋白质的翻译后共价修饰。
Science. 1977 Dec 2;198(4320):890-6. doi: 10.1126/science.337487.
9
Evidence for covalent attachment of fatty acids to Sindbis virus glycoproteins.脂肪酸与辛德毕斯病毒糖蛋白共价连接的证据。
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1687-91. doi: 10.1073/pnas.76.4.1687.
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Adenosine 3':5'-monophosphate dependent protein kinase from bovine heart. Characterization of the catalytic subunit.
Biochemistry. 1977 Dec 27;16(26):5691-7. doi: 10.1021/bi00645a007.