Scott P, James S, Sher A
Eur J Immunol. 1985 Jun;15(6):553-8. doi: 10.1002/eji.1830150605.
The macrophage cell line, IC-21, was found to be incapable of producing the oxygen products associated with the respiratory burst. However, IC-21 cells were activated by lymphokine (LK) to kill intracellular (Leishmania donovani amastigotes) and extracellular (Schistosoma mansoni larvae) parasites, as well as tumor cells. In each case, the cytotoxicity exhibited by activated IC-21 cells and activated peritoneal macrophages was indistinguishable. However, nonactivated IC-21 cells were unable to kill L. donovani log-growth phase promastigotes, while nonactivated peritoneal macrophages destroyed greater than 90% of the initial infection. These results indicate that amastigotes and schistosome larvae are susceptible to killing by nonoxidative cytotoxic mechanism induced by lymphokine activation but, on the other hand, support the concept that the killing of log-growth phase promastigotes by nonactivated cells is dependent upon the respiratory burst. We propose that the IC-21 cell line may be a useful model for studying nonoxidative killing functions of activated macrophages.
巨噬细胞系IC-21被发现无法产生与呼吸爆发相关的氧产物。然而，IC-21细胞可被淋巴因子（LK）激活，从而杀死细胞内的（杜氏利什曼原虫无鞭毛体）和细胞外的（曼氏血吸虫幼虫）寄生虫以及肿瘤细胞。在每种情况下，活化的IC-21细胞和活化的腹腔巨噬细胞所表现出的细胞毒性并无差异。然而，未活化的IC-21细胞无法杀死处于对数生长期的杜氏利什曼原虫前鞭毛体，而未活化的腹腔巨噬细胞则可破坏超过90%的初始感染。这些结果表明，无鞭毛体和血吸虫幼虫易被淋巴因子激活诱导的非氧化细胞毒性机制所杀伤，但另一方面，也支持了未活化细胞对对数生长期前鞭毛体的杀伤依赖于呼吸爆发这一观点。我们认为，IC-21细胞系可能是研究活化巨噬细胞非氧化杀伤功能的有用模型。
