• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人唾液腺来源的初代上皮细胞的特性及其对类腺泡结构形成的影响。

Characterization of Primary Epithelial Cells Derived from Human Salivary Gland Contributing to Formation of Acini-like Structures.

机构信息

Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University, Seoul 06351, Korea.

Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Korea.

出版信息

Mol Cells. 2018 Jun;41(6):515-522. doi: 10.14348/molcells.2018.0060. Epub 2018 Jun 11.

DOI:10.14348/molcells.2018.0060
PMID:29890826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6030237/
Abstract

Patients with head and neck cancer are treated with therapeutic irradiation, which can result in irreversible salivary gland dysfunction. Because there is no complete cure for such patients, stem cell therapy is an emerging alternative for functional restoration of salivary glands. In this study, we investigated characteristics of primarily isolated epithelial cells from human salivary gland (Epi-SGs) and formation of acini-like structures by Epi-SGs. Primarily isolated Epi-SGs showed typical epithelial cell-like morphology and expressed E-cadherin but not N-cadherin. Epi-SGs expressed epithelial stem cell (EpiSC) and embryonic stem cell (ESC) markers. During long-term culture, the expression of EpiSC and ESC markers was highly detected and maintained within the core population with small size and low cytoplasmic complexity. The core population expressed cytokeratin 7 and cytokeratin 14, known as duct markers indicating that Epi-SGs might be originated from the duct. When Epi-SGs were transplanted with Matrigel, acini-like structures were readily formed at 4 days after transplantation and they were maintained at 7 days after transplantation. Taken together, our data suggested that Epi-SGs might contain stem cells which were positive for EpiSC and ESC markers, and Epi-SGs might contribute to the regeneration of acini-like structures . We expect that Epi-SGs will be useful source for the functional restoration of damaged salivary gland.

摘要

头颈部癌症患者接受治疗性放疗，这可能导致不可逆转的唾液腺功能障碍。由于此类患者没有完全治愈的方法，因此干细胞治疗是恢复唾液腺功能的一种新兴替代方法。在这项研究中，我们研究了人唾液腺（Epi-SG）中最初分离的上皮细胞的特征以及 Epi-SG 形成类腺泡结构的特性。最初分离的 Epi-SG 表现出典型的上皮细胞样形态，并表达 E-钙粘蛋白，但不表达 N-钙粘蛋白。Epi-SG 表达上皮干细胞（EpiSC）和胚胎干细胞（ESC）标志物。在长期培养中，EpiSC 和 ESC 标志物的表达在具有小尺寸和低细胞质复杂性的核心群体中高度检测到并维持。核心群体表达角蛋白 7 和角蛋白 14，这是导管标志物，表明 Epi-SG 可能来源于导管。当 Epi-SG 与 Matrigel 一起移植时，在移植后 4 天就可以很容易地形成类腺泡结构，并且在移植后 7 天仍能维持。总之，我们的数据表明，Epi-SG 可能含有干细胞，这些干细胞对 EpiSC 和 ESC 标志物呈阳性，Epi-SG 可能有助于类腺泡结构的再生。我们期望 Epi-SG 将成为受损唾液腺功能恢复的有用来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/153478736d07/molce-41-6-515f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/ea178c269568/molce-41-6-515f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/5a3076c67cb2/molce-41-6-515f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/6050f3035063/molce-41-6-515f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/153478736d07/molce-41-6-515f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/ea178c269568/molce-41-6-515f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/5a3076c67cb2/molce-41-6-515f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/6050f3035063/molce-41-6-515f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb10/6030237/153478736d07/molce-41-6-515f4.jpg

相似文献

1
Characterization of Primary Epithelial Cells Derived from Human Salivary Gland Contributing to Formation of Acini-like Structures.人唾液腺来源的初代上皮细胞的特性及其对类腺泡结构形成的影响。
Mol Cells. 2018 Jun;41(6):515-522. doi: 10.14348/molcells.2018.0060. Epub 2018 Jun 11.
2
Intraglandular transplantation of bone marrow-derived clonal mesenchymal stem cells for amelioration of post-irradiation salivary gland damage.骨髓源性克隆间充质干细胞经腺体内移植改善放射性唾液腺损伤。
Oral Oncol. 2013 Feb;49(2):136-43. doi: 10.1016/j.oraloncology.2012.08.010. Epub 2012 Sep 13.
3
Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation.骨髓来源的细胞可挽救头颈部放疗小鼠的唾液腺功能。
Int J Biochem Cell Biol. 2011 Jan;43(1):80-7. doi: 10.1016/j.biocel.2010.09.023. Epub 2010 Oct 7.
4
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: role of TGF-β1 in salivary gland epithelial and mesenchymal differentiation.用于唾液腺上皮细胞和间充质细胞长期扩增的体外培养系统：TGF-β1 在唾液腺上皮细胞和间充质分化中的作用。
Biomed Res Int. 2013;2013:815895. doi: 10.1155/2013/815895. Epub 2013 Jun 9.
5
Induction of Salivary Gland-Like Cells from Dental Follicle Epithelial Cells.从牙囊上皮细胞诱导唾液腺样细胞
J Dent Res. 2017 Aug;96(9):1035-1043. doi: 10.1177/0022034517711146. Epub 2017 May 25.
6
Diverse epithelial cell populations contribute to the regeneration of secretory units in injured salivary glands.多种上皮细胞群体有助于受损唾液腺分泌单位的再生。
Development. 2020 Oct 9;147(19):dev192807. doi: 10.1242/dev.192807.
7
Induction of salivary gland-like cells from epithelial tissues transdifferentiated from mouse embryonic fibroblasts.从鼠胚胎成纤维细胞转分化的上皮组织中诱导产生唾液腺样细胞。
Biochem Biophys Res Commun. 2022 Jan 1;586:55-62. doi: 10.1016/j.bbrc.2021.11.064. Epub 2021 Nov 19.
8
Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands.外周血单个核细胞的抗炎和血管生成条件作用增强了其治疗辐射损伤唾液腺的治疗潜力。
Stem Cell Res Ther. 2019 Oct 17;10(1):304. doi: 10.1186/s13287-019-1414-7.
9
Protective efficacy of intravenous transplantation of adipose-derived stem cells for the prevention of radiation-induced salivary gland damage.静脉注射脂肪来源干细胞预防放射性唾液腺损伤的保护效果
Arch Oral Biol. 2015 Oct;60(10):1488-96. doi: 10.1016/j.archoralbio.2015.07.016. Epub 2015 Jul 29.
10
Immunocytochemistry of myoepithelial cells in the salivary glands.唾液腺肌上皮细胞的免疫细胞化学
Prog Histochem Cytochem. 2003;38(4):343-426. doi: 10.1016/s0079-6336(03)80001-3.

引用本文的文献

1
CD9-enriched extracellular vesicles from chemically reprogrammed basal progenitors of salivary glands mitigate salivary gland fibrosis.来自化学重编程唾液腺基底祖细胞的富含CD9的细胞外囊泡可减轻唾液腺纤维化。
Bioact Mater. 2025 Jan 24;47:229-247. doi: 10.1016/j.bioactmat.2025.01.019. eCollection 2025 May.
2
The Alteration of Salivary Immunoglobulin A in Autism Spectrum Disorders.自闭症谱系障碍患者唾液免疫球蛋白A的改变
Front Psychiatry. 2021 May 21;12:669193. doi: 10.3389/fpsyt.2021.669193. eCollection 2021.
3
Immortalization of Salivary Gland Epithelial Cells of Xerostomic Patients: Establishment and Characterization of Novel Cell Lines.

本文引用的文献

1
Concise Review: Salivary Gland Regeneration: Therapeutic Approaches from Stem Cells to Tissue Organoids.简明综述：唾液腺再生：从干细胞到组织类器官的治疗方法
Stem Cells. 2017 Jan;35(1):97-105. doi: 10.1002/stem.2455. Epub 2016 Jul 15.
2
Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands.人唾液腺干细胞可功能性修复受辐射损伤的唾液腺。
Stem Cells. 2016 Mar;34(3):640-52. doi: 10.1002/stem.2278.
3
Purification and ex vivo expansion of fully functional salivary gland stem cells.唾液腺干细胞的纯化和体外扩增。
口干燥症患者唾液腺上皮细胞的永生化：新型细胞系的建立与鉴定
J Clin Med. 2020 Nov 25;9(12):3820. doi: 10.3390/jcm9123820.
4
An In Vitro Barrier Model of the Human Submandibular Salivary Gland Epithelium Based on a Single Cell Clone of Cell Line HTB-41: Establishment and Application for Biomarker Transport Studies.基于细胞系HTB-41单细胞克隆的人下颌下唾液腺上皮体外屏障模型：生物标志物转运研究的建立与应用
Biomedicines. 2020 Aug 23;8(9):302. doi: 10.3390/biomedicines8090302.
5
Building a Functional Salivary Gland for Cell-Based Therapy: More than Secretory Epithelial Acini.构建基于细胞治疗的功能性唾液腺：不仅仅是分泌上皮细胞。
Tissue Eng Part A. 2020 Dec;26(23-24):1332-1348. doi: 10.1089/ten.TEA.2020.0184. Epub 2020 Sep 21.
Stem Cell Reports. 2014 Dec 9;3(6):957-64. doi: 10.1016/j.stemcr.2014.09.015. Epub 2014 Oct 23.
4
Heterogeneity and hierarchy of hematopoietic stem cells.造血干细胞的异质性和层级性。
Exp Hematol. 2014 Feb;42(2):74-82.e2. doi: 10.1016/j.exphem.2013.11.004. Epub 2013 Nov 20.
5
Capability of tissue stem cells to organize into salivary rudiments.组织干细胞形成唾液腺原基的能力。
Stem Cells Int. 2012;2012:502136. doi: 10.1155/2012/502136. Epub 2012 Mar 15.
6
Epithelial cell adhesion molecule (EpCAM) complex proteins promote transcription factor-mediated pluripotency reprogramming.上皮细胞黏附分子（EpCAM）复合物蛋白促进转录因子介导的多能性重编程。
J Biol Chem. 2011 Sep 23;286(38):33520-32. doi: 10.1074/jbc.M111.256164. Epub 2011 Jul 28.
7
Epithelial stem/progenitor cells in the embryonic mouse submandibular gland.胚胎期小鼠下颌下腺中的上皮干细胞/祖细胞。
Front Oral Biol. 2010;14:90-106. doi: 10.1159/000313709. Epub 2010 Apr 20.
8
Evidence of an epithelial stem/progenitor cell hierarchy in the adult mouse lung.成年小鼠肺中的上皮干细胞/祖细胞层级证据。
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1414-9. doi: 10.1073/pnas.0909207107. Epub 2010 Jan 4.
9
Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.通过联合膜-多核糖体翻译状态阵列分析和免疫转录谱分析鉴定人胚胎干细胞表面标志物。
Stem Cells. 2009 Oct;27(10):2446-56. doi: 10.1002/stem.182.
10
Isolation and characterization of human salivary gland cells for stem cell transplantation to reduce radiation-induced hyposalivation.用于减少辐射性口干的干细胞移植的人唾液腺细胞的分离和鉴定。
Radiother Oncol. 2009 Sep;92(3):466-71. doi: 10.1016/j.radonc.2009.06.023. Epub 2009 Jul 20.