与果蝇核糖体蛋白基因具有同源性的小鼠DNA片段的分离。

Isolation of a mouse DNA fragment with homology to a Drosophila ribosomal protein gene.

作者信息

Mulligan P K, Hackett P B

出版信息

Gene. 1985;34(2-3):155-61. doi: 10.1016/0378-1119(85)90123-4.

Abstract

A mouse genomic library in lambda Charon 4A was screened for putative ribosomal protein genes using a fragment of the gene encoding Drosophila ribosomal protein 49 as a hybridization probe under nonstringent hybridization conditions. A recombinant phage was selected and its restriction enzyme map determined. The major species of mouse poly(A)+ mRNA homologous to the putative gene is about 740 nucleotides long.

摘要

利用编码果蝇核糖体蛋白49的基因片段作为杂交探针，在非严格杂交条件下，对λ噬菌体Charon 4A载体构建的小鼠基因组文库进行筛选，以寻找假定的核糖体蛋白基因。挑选出一个重组噬菌体，并确定其限制酶图谱。与该假定基因同源的小鼠多聚腺苷酸（poly(A)+）mRNA的主要种类约为740个核苷酸长。