Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica - CeTroGen, Hospital Universitário Maria Aparecida Pedrossian - HUMAP, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brazil; Programa de Pós-graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta" - FAMED, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brazil.
Programa de Pós-graduação em Química - INQUI, Universidade Federal de Mato Grosso do Sul- UFMS, Campo Grande, MS, Brazil; Laboratório de Combustiveis - LABCOM, Instituto de Química - INQUI, Universidade Federal de Mato Grosso do Sul- UFMS, Campo Grande, MS, Brazil.
Pestic Biochem Physiol. 2018 Jun;148:22-27. doi: 10.1016/j.pestbp.2018.03.010. Epub 2018 Mar 26.
Temephos is considered the gold standard by the Ministry of Health for controlling the larvae of the mosquito Aedes aegypti. The present study evaluated the effects of Temephos larvicide on the reproductive performance, embryo-fetal development and DNA integrity of Swiss mice. This study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 mL/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with Temephos at doses of 0.0043 mg/kg and 0.043 mg/kg (b.w., a.o.) during the gestational period. Statistical analysis showed that Temephos did not alter the biometric or reproductive parameters. Comparing the weight of the fetus to the stage of pregnancy demonstrated that the 0.0043 mg/kg dosage increased the size of the fetuses. No external malformations were detected. However, the 0.043 mg/kg dosage induced changes in the sternum, with the main change being the center of the sternum, xiphoid processes and absence of the manubrium. The other skeletal and visceral alterations did not differ from the control group and are considered variants of normality. The analysis of head measurements showed an increase in the anterior/posterior measurements of the glabella, the external occipital protuberance and the biauricular plane. The circumference and area of the head did not present significant differences. The micronucleus test showed only a 0.043 mg/kg increase in 48 h. Thus, it is considered that Temephos has a low teratogenic and genotoxic risk.
马拉硫磷被卫生部视为控制埃及伊蚊幼虫的金标准。本研究评估了马拉硫磷杀幼虫剂对瑞士小鼠生殖性能、胚胎-胎儿发育和 DNA 完整性的影响。本研究使用了 30 只怀孕雌性小鼠：10 只为对照组，用 0.1ml/10g（体重- b.w.，口服- a.o.）的饮用水处理，20 只为实验组，在妊娠期用 0.0043mg/kg 和 0.043mg/kg（b.w.，a.o.）的马拉硫磷处理。统计分析表明，马拉硫磷并未改变生物计量或生殖参数。比较胎儿体重与妊娠阶段表明，0.0043mg/kg 剂量增加了胎儿的大小。未发现外部畸形。然而，0.043mg/kg 剂量引起胸骨变化，主要变化是胸骨中心、剑突和胸骨柄缺失。其他骨骼和内脏变化与对照组没有区别，被认为是正常变异。头部测量分析显示，额骨、枕外隆突和双耳平面的前后测量值增加。头部的周长和面积没有显著差异。微核试验仅在 48 小时显示 0.043mg/kg 增加。因此，认为马拉硫磷具有较低的致畸和遗传毒性风险。
