Shih Miao-Ling, López-González Ma de Lourdes, Uribe-Ramírez Marisela, Rojas-García Aurora Elizabeth, Verdín-Betancourt Francisco Alberto, Sierra-Santoyo Adolfo
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, Ciudad de México 07360, Mexico.
Laboratorio de Contaminación y Toxicología Ambiental, Secretaría de Investigación y Posgrado, Universidad Autónoma de Nayarit, Tepic 63000, Nay., Mexico.
J Xenobiot. 2024 Dec 4;14(4):1889-1900. doi: 10.3390/jox14040100.
Temephos is an organophosphorus pesticide widely used as a larvicide in public health campaigns to control vector-borne diseases. Data on the urinary elimination of temephos metabolites are limited, and there is no validated biomarker of exposure for its evaluation. This study aimed to determine the urinary excretion kinetics of temephos and its metabolites in adult male rats. Hence, adult male Wistar rats were administered orally with a single dose of temephos (300 mg/kg). Urine samples were collected at different time intervals after dosing and enzymatically hydrolyzed using β-glucuronidase/sulfatase from . The metabolites were extracted and analyzed by HPLC-DAD. The metabolites detected were 4,4'-thiodiphenol (TDP), 4,4'-sulfinyldiphenol (SIDP), 4,4'-sulfonyldiphenol (SODP), or bisphenol S (BPS), a non-identified metabolite, and only traces of the parent compound. The mean urine concentrations of metabolites were used for kinetic analysis. Urinary levels of TDP were fitted to a two-compartmental model, and its half-lives () were 27.8 and 272.1 h for the first and second phases, respectively. The of BPS was 17.7 h. TDP, the main metabolite of temephos, was eliminated by urine and is specific and stable. Therefore, it may be used as a biomarker of temephos exposure.
双硫磷是一种有机磷杀虫剂，在公共卫生运动中广泛用作杀幼虫剂，以控制病媒传播疾病。关于双硫磷代谢物经尿液排泄的数据有限，且尚无经过验证的用于评估其暴露情况的生物标志物。本研究旨在确定成年雄性大鼠体内双硫磷及其代谢物的尿排泄动力学。因此，给成年雄性Wistar大鼠口服单剂量双硫磷（300毫克/千克）。给药后在不同时间间隔收集尿液样本，并使用来自[具体来源未给出]的β-葡萄糖醛酸酶/硫酸酯酶进行酶促水解。代谢物经提取后用高效液相色谱-二极管阵列检测法（HPLC-DAD）进行分析。检测到的代谢物有4,4'-硫代二苯酚（TDP）、4,4'-亚磺酰基二苯酚（SIDP）、4,4'-磺酰基二苯酚（SODP）或双酚S（BPS，一种未鉴定的代谢物），且仅检测到痕量的母体化合物。代谢物的平均尿液浓度用于动力学分析。TDP的尿液水平拟合为二室模型，其第一相和第二相的半衰期分别为27.8小时和272.1小时。BPS的半衰期为17.7小时。双硫磷的主要代谢物TDP经尿液排泄，具有特异性且稳定。因此，它可作为双硫磷暴露的生物标志物。
