Akbarian Naghmeh, Zarghami Nosratollah, Mota Ali, Abediazar Sima, Abroon Sina, Mihanfar Aynaz, Amanzadeh Mina, Darbin Akbar, Bannazadeh Baghi Hossein, Rahmati-Yamchi Mohammad
Drug Applied Research Center; Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences; Tabriz, Iran.
Iran J Kidney Dis. 2018 May;12(3):163-168.
Diabetic nephropathy is one of the serious complications of diabetes mellitus. Visfatin is an intracellular enzyme with insulin-mimicking effects. It enhances the expression of endothelial nitric oxide (NO) synthase in renal cells. This study aimed to investigate serum levels of visfatin and NO metabolites in patients with diabetic nephropathy.
A total of 80 diabetic patients were enrolled and classified into nephropathic and non-nephropathic patients. Serum visfatin and insulin levels were estimated using an enzyme-linked immunosorbent assay, and NO metabolites were estimated using a colorimetric assay.
Serum visfatin and NO metabolites levels were significantly elevated in the patients with diabetic nephropathy. Serum visfatin levels and NO metabolites were significantly higher in the nephropathic patients (P = .003; 95% confidence interval, 2.29 to 10.81; P < .001; 95% confidence interval, 3.14 to 9.46, respectively) as compared to the control group, whereas homeostatic model assessment-insulin resistance was significantly lower (P = .02; 95% confidence interval, -1.51 to -1.01).There was no correlation between body mass index, blood pressure, lipid profile, insulin, and glucose levels and serum visfatin and NO metabolites levels.
The results of this study demonstrated that there were high levels of visfatin and NO metabolites in patients with diabetic nephropathy. In addition, there was a positive correlation between visfatin and NO metabolites levels in nephropathic and non-nephropathic diabetic patients.
糖尿病肾病是糖尿病的严重并发症之一。内脂素是一种具有胰岛素模拟作用的细胞内酶。它可增强肾细胞中内皮型一氧化氮合酶的表达。本研究旨在调查糖尿病肾病患者血清内脂素水平和一氧化氮代谢产物水平。
共纳入80例糖尿病患者，并分为肾病组和非肾病组。采用酶联免疫吸附测定法评估血清内脂素和胰岛素水平，采用比色法评估一氧化氮代谢产物水平。
糖尿病肾病患者血清内脂素和一氧化氮代谢产物水平显著升高。与对照组相比，肾病组患者血清内脂素水平和一氧化氮代谢产物水平显著更高（P = 0.003；95%置信区间，2.29至10.81；P < 0.001；95%置信区间，分别为3.14至9.46），而稳态模型评估胰岛素抵抗显著更低（P = 0.02；95%置信区间，-1.51至-1.01）。体重指数、血压、血脂谱、胰岛素和血糖水平与血清内脂素和一氧化氮代谢产物水平之间无相关性。
本研究结果表明，糖尿病肾病患者内脂素和一氧化氮代谢产物水平较高。此外，肾病组和非肾病组糖尿病患者的内脂素和一氧化氮代谢产物水平呈正相关。
