Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea.
Department of Life Systems, Sookmyung Women's University, Cheongpa-ro 47-gil 100, Yongsan-gu, Seoul 04310, Korea.
Nutrients. 2018 Jun 11;10(6):754. doi: 10.3390/nu10060754.
Thunberg is an herbal medicine used to treat neuralgia, rheumatoid arthritis, and inflammatory-related diseases. However, its effects on osteoarthritis (OA) and its regulatory mechanisms have not been investigated by network analysis. Here, we investigated the pharmacological effects of extract (PJE) on OA, by combining in vivo effective verification and network pharmacology prediction. Rats in which OA was induced by monosodium iodoacetate (MIA) were treated with PJE (200 mg/kg), and histopathological parameters, weight bearing distribution and inflammatory factors in serum and joint tissue were measured after 28 days of treatment. Additionally, in silico network analysis was used to predict holistic OA regulatory mechanisms of PJE. The results showed that PJE exerted potential protective effects by recovering hind paw weight bearing distribution, alleviating histopathological features of cartilage and inhibiting inflammatory mediator levels in the OA rat model. Furthermore, network analysis identified caspase-3 (CASP3), caspase-7 (CASP7), and cytochrome P450 2D6 (CYP2D6) as potential target genes; in addition, the TNF (Tumor necrosis factor) signaling pathway was linked to OA therapeutic action. Our combined animal OA model and network analysis confirmed the therapeutic effects of PJE against OA and identified intracellular signaling pathways, active compounds and target genes linked to its therapeutic action.
白皮杉醇是一种草药，用于治疗神经痛、类风湿性关节炎和炎症相关疾病。然而，其对骨关节炎（OA）的作用及其调节机制尚未通过网络分析进行研究。在这里，我们通过结合体内有效验证和网络药理学预测，研究了白皮杉醇提取物（PJE）对 OA 的药理作用。用单碘乙酸（MIA）诱导 OA 的大鼠用 PJE（200 mg/kg）治疗 28 天后，测量其血清和关节组织中的组织病理学参数、负重分布和炎症因子。此外，还使用计算网络分析来预测 PJE 对整体 OA 的调节机制。结果表明，PJE 通过恢复后爪负重分布、减轻软骨组织的组织病理学特征和抑制 OA 大鼠模型中炎症介质水平，发挥潜在的保护作用。此外，网络分析确定了半胱天冬酶-3（CASP3）、半胱天冬酶-7（CASP7）和细胞色素 P450 2D6（CYP2D6）作为潜在的靶基因；此外，TNF（肿瘤坏死因子）信号通路与 OA 的治疗作用有关。我们的动物 OA 模型和网络分析的组合证实了 PJE 对 OA 的治疗作用，并确定了与治疗作用相关的细胞内信号通路、活性化合物和靶基因。
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