Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Department of Biomedical Engineering, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.
Sci Rep. 2018 Jun 11;8(1):8875. doi: 10.1038/s41598-018-27130-9.
Notochordal cell derived matrix (NCM) can induce regenerative effects on nucleus pulposus cells and may exert such effects on chondrocytes as well. Furthermore, when dissolved at low concentrations, NCM forms a viscous fluid with potential lubricating properties. Therefore, this study tests the feasibility of the use of NCM as a regenerative lubricant for the osteoarthritic joint. Chondrocyte-seeded alginate beads were cultured in base medium (BM), BM with NCM (NCM), or BM with TGF-β1 (TGF), as well as BM and NCM treated with IL-1β. NCM increased GAG deposition and cell proliferation (stronger than TGF), and GAG/DNA ratio and hydroxyproline content (similar to TGF). These effects were maintained in the presence of IL-1β. Moreover, NCM mitigated expression of IL-1β-induced IL-6, IL-8, ADAMTS-5 and MMP-13. Reciprocating sliding friction tests of cartilage on glass were performed to test NCM's lubricating properties relative to hyaluronic acid (HA), and showed a dose-dependent reduction in coefficient of friction with NCM, similar to HA. NCM has anabolic and anti-inflammatory effects on chondrocytes, as well as lubricating properties. Therefore, intra-articular NCM injection may have potential as a treatment to minimize pain while restoring the affected cartilage tissue in the osteoarthritic joint.
脊索细胞衍生基质 (NCM) 可对髓核细胞产生再生作用，并且可能对软骨细胞也有类似作用。此外，当以低浓度溶解时，NCM 形成具有潜在润滑特性的粘性流体。因此，本研究测试了将 NCM 用作骨关节炎关节再生润滑剂的可行性。将软骨细胞接种的藻酸盐珠在基础培养基 (BM)、含 NCM 的 BM (NCM) 或含 TGF-β1 的 BM (TGF) 中培养，以及在 BM 和 NCM 中用 IL-1β 处理。NCM 增加了 GAG 沉积和细胞增殖（比 TGF 更强），并且 GAG/DNA 比和羟脯氨酸含量（与 TGF 相似）。在存在 IL-1β 的情况下，这些作用得以维持。此外，NCM 减轻了 IL-1β 诱导的 IL-6、IL-8、ADAMTS-5 和 MMP-13 的表达。进行了软骨在玻璃上的往复滑动摩擦测试，以测试 NCM 相对于透明质酸 (HA) 的润滑性能，结果表明 NCM 的摩擦系数呈剂量依赖性降低，与 HA 相似。NCM 对软骨细胞具有合成代谢和抗炎作用，并且具有润滑特性。因此，关节内注射 NCM 可能具有潜力，可在减轻疼痛的同时恢复骨关节炎关节中受影响的软骨组织。
