Akhbari Maryam, Masoum Saeed, Aghababaei Fahimeh, Hamedi Sepideh
1Essential Oils Research Institute, University of Kashan, Kashan, Iran.
2Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.
J Food Sci Technol. 2018 Jun;55(6):2197-2207. doi: 10.1007/s13197-018-3137-7. Epub 2018 Mar 24.
In this study, the efficiencies of conventional hydro-distillation and novel microwave hydro-distillation methods in extraction of essential oil from leaves have been compared. In order to attain the best yield and also highest quality of the essential oil in the microwave assisted method, the optimal values of operating parameters such as extraction time, microwave irradiation power and water volume to plant mass ratio were investigated using central composite design under response surface methodology. Optimal conditions for obtaining the maximum extraction yield in the microwave assisted method were predicted as follows: extraction time of 85 min, microwave power of 888 W, and water volume to plant mass ratio of 0.5 ml/g. The extraction yield at these predicted conditions was computed as 0.7756%. The qualities of the obtained essential oils under designed experiments were optimized based on total contents of four major compounds (α-pinene, 1,8-cineole, camphor and verbenone) which determined by gas chromatography equipped with mass spectroscopy (GC-MS). The highest essential oil quality (55.87%) was obtained at extraction time of 68 min; microwave irradiation power of 700 W; and water volume to plant mass ratio of zero.
在本研究中，对传统水蒸馏法和新型微波水蒸馏法从叶片中提取精油的效率进行了比较。为了在微波辅助法中获得最佳产率和最高品质的精油，采用响应面法中的中心复合设计，研究了提取时间、微波辐射功率和水与植物质量比等操作参数的最佳值。微波辅助法获得最大提取产率的最佳条件预测如下：提取时间85分钟、微波功率888瓦、水与植物质量比0.5毫升/克。在这些预测条件下的提取产率计算为0.7756%。基于配备质谱仪的气相色谱法（GC-MS）测定的四种主要化合物（α-蒎烯、1,8-桉叶素、樟脑和马鞭草烯酮）的总含量，对设计实验中获得的精油品质进行了优化。在提取时间68分钟、微波辐射功率700瓦和水与植物质量比为零的条件下，获得了最高的精油品质（55.87%）。
