内切-β-N-乙酰氨基葡萄糖苷酶突变体内切-CC N180H的受体范围：从单糖到抗体。

Manabe Shino, Yamaguchi Yoshiki, Abe Junpei, Matsumoto Kana, Ito Yukishige

Synthetic Cellular Chemistry Laboratory, RIKEN, Hirosawa, Wako, Saitama 351-0198, Japan.

Structural Glycobiology Team, RIKEN, Hirosawa, Wako, Saitama 351-0198, Japan.

R Soc Open Sci. 2018 May 16;5(5):171521. doi: 10.1098/rsos.171521. eCollection 2018 May.

The endo-β--acetylglucosaminidase mutant endo-CC N180H transfers glycan from sialylglycopeptide (SGP) to various acceptors. The scope and limitations of low-molecular-weight acceptors were investigated. Several homogeneous glycan-containing compounds, especially those with potentially useful labels or functional moieties, and possible reagents in glycoscience were synthesized. The 1,3-diol structure is important in acceptor molecules in glycan transfer reactions mediated by endo-CC N180H as well as by endo-M-N175Q. Glycan remodelling of antibodies was explored using core-fucose-deficient anti-CCR4 antibody with SGP and endo-CC N180H. Homogeneity of the glycan in the antibody was confirmed by mass spectrometry without glycan cleavage.

内切-β-N-乙酰氨基葡萄糖苷酶突变体内切-CC N180H可将聚糖从唾液酸化糖肽（SGP）转移至各种受体。研究了低分子量受体的适用范围和局限性。合成了几种均一的含聚糖化合物，尤其是那些带有潜在有用标记或功能基团的化合物，以及糖科学中可能用到的试剂。在由内切-CC N180H以及内切-M-N175Q介导的聚糖转移反应中，1,3-二醇结构在受体分子中很重要。利用缺乏核心岩藻糖的抗CCR4抗体、SGP和内切-CC N180H探索了抗体的聚糖重塑。通过质谱法在不进行聚糖切割的情况下确认了抗体中聚糖的均一性。