San Millán J L, Kolter R, Moreno F
J Bacteriol. 1985 Sep;163(3):1016-20. doi: 10.1128/jb.163.3.1016-1020.1985.
The production of the antibiotic substance microcin B17 (Mcc) is determined by a 3.5-kilobase DNA fragment from plasmid pMccB17. Several Mcc- mutations on plasmid pMccB17 were obtained by both transposon insertion and nitrosoguanidine mutagenesis. Plasmids carrying these mutations were tested for their ability to complement Mcc- insertion or deletion mutations on pMM102 (pMM102 is a pBR322 derivative carrying the region encoding microcin B17). Results from these experiments indicate that at least four plasmid genes are required for microcin production.
抗生素物质微菌素B17（Mcc）的产生由质粒pMccB17上一个3.5千碱基的DNA片段决定。通过转座子插入和亚硝基胍诱变在质粒pMccB17上获得了几个Mcc - 突变体。测试携带这些突变的质粒对pMM102（pMM102是携带编码微菌素B17区域的pBR322衍生物）上Mcc - 插入或缺失突变的互补能力。这些实验结果表明，微菌素产生至少需要四个质粒基因。
