Bernal Juan, Morte Beatriz
Instituto de Investigaciones Biomedicas, Consejo Superior de Investigaciones Científicas (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain.
Center for Biomedical Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III, Madrid, Spain.
Methods Mol Biol. 2018;1801:17-28. doi: 10.1007/978-1-4939-7902-8_3.
The actions of thyroid hormones on brain development and function are due primarily to regulation of gene expression. Identification of direct transcriptional responses requires cell culture approaches given the difficulty of in vivo studies. Here, we describe the use of primary cells in culture obtained from embryonic mouse cerebral cortex, to identify the set of genes regulated directly and indirectly by T3 using RNA-Seq.
甲状腺激素对大脑发育和功能的作用主要归因于基因表达的调控。鉴于体内研究的困难，直接转录反应的鉴定需要细胞培养方法。在这里，我们描述了使用从胚胎小鼠大脑皮层获得的原代培养细胞，通过RNA测序来鉴定由T3直接和间接调控的基因集。
