Lee Jae Chul
Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-Gil, Bundang-Gu, Seongnam-si, Gyeonggi-do, 463707, Republic of Korea.
Genes Genomics. 2018 Jan;40(1):25-37. doi: 10.1007/s13258-017-0607-7. Epub 2017 Sep 7.
Osteoarthritis (OA) is a degenerative joint disease characterized by abrasion, and ultimately, destruction of the articular cartilage and trabecular bone loss. OA is still considered a devastating disease, which requires an aggressive therapeutic approach. Despite the therapeutic potential of human adipose-derived mesenchymal stem cells (AD-MSCs), the molecular parameters needed to define the "stemness" remain largely unknown. Using high-density oligonucleotide microarrays, the differential gene expression profiles between a fraction of human adipose-derived (AD) mononuclear cells and its MSC subpopulation were obtained. Of particular interest was a subset of 58 genes preferentially expressed at sevenfold or higher in the group treated with human AD-MSCs. This subset contained numerous genes involved in the inflammatory response, immune response, lipid metabolism, cell death, cell proliferation, and DNA repair. Additionally, four protein networks were constructed. The interaction network consisted of 46 proteins encoded by up-regulated genes. However, the interaction network also consisted of 38 proteins encoded by down-regulated genes. My results provide a basis for a more reproducible and reliable quality control using genotypic analysis for the definition of human AD-MSCs. Therefore, these results will provide a basis for studies on molecular mechanisms controlling the core properties of human MSCs.
骨关节炎（OA）是一种退行性关节疾病，其特征为磨损，最终导致关节软骨磨损以及小梁骨丢失。OA仍被认为是一种破坏性疾病，需要积极的治疗方法。尽管人脂肪来源的间充质干细胞（AD-MSCs）具有治疗潜力，但定义“干性”所需的分子参数在很大程度上仍不清楚。使用高密度寡核苷酸微阵列，获得了人脂肪来源（AD）的单核细胞部分与其MSC亚群之间的差异基因表达谱。特别令人感兴趣的是一组58个基因的子集，这些基因在用人AD-MSCs处理的组中以七倍或更高的倍数优先表达。该子集包含许多参与炎症反应、免疫反应、脂质代谢、细胞死亡、细胞增殖和DNA修复的基因。此外，构建了四个蛋白质网络。相互作用网络由上调基因编码的46种蛋白质组成。然而，相互作用网络也由下调基因编码的38种蛋白质组成。我的结果为使用基因型分析定义人AD-MSCs提供了更可重复和可靠的质量控制基础。因此，这些结果将为研究控制人MSC核心特性的分子机制提供基础。
