Singer Y, Kimmel N, Sarov I
J Virol Methods. 1985 May;11(1):29-39. doi: 10.1016/0166-0934(85)90122-3.
A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase conjugate was used to detect IgM bound to viral antigen. In parallel the enzyme linked immunosorbent assay (ELISA) technique was used to determine IgG and IgM antibodies to CMV, respectively. All patients with acute CMV infections who were tested had CMV-specific IgM antibodies by IPA, both whole cell and nuclei antigen. The maximal IgM titers were higher by ELISA than by IPA but in 3 of the CMV patients IgM was detected earlier by IPA (with both types of antigens) than by ELISA. In 3 of 5 transplant patients with recurrent CMV infection IgM was demonstrated by immunoperoxidase techniques, while by ELISA IgM was demonstrated in only 2 of them. No cross reactivity with other herpes viruses was observed. The described IPA is simple, rapid and has the potential for widespread use in routine laboratories.
本文描述了一种适用于检测血清中抗巨细胞病毒（CMV）IgM抗体的简易免疫过氧化物酶测定法（IPA）。抗原由CMV感染的人胚成纤维细胞或分离的细胞核组成。检测前血清用聚合γ球蛋白吸收。兔抗人IgM过氧化物酶结合物用于检测与病毒抗原结合的IgM。同时采用酶联免疫吸附测定（ELISA）技术分别测定抗CMV的IgG和IgM抗体。所有接受检测的急性CMV感染患者通过IPA检测全细胞和细胞核抗原时均有CMV特异性IgM抗体。ELISA法检测到的最大IgM滴度高于IPA法，但在3例CMV患者中，IPA法（两种抗原类型）比ELISA法更早检测到IgM。5例复发性CMV感染的移植患者中，3例通过免疫过氧化物酶技术检测到IgM，而ELISA法仅在其中2例中检测到IgM。未观察到与其他疱疹病毒的交叉反应。所描述的IPA方法简单、快速，有在常规实验室广泛应用的潜力。
