Cappel R, de Cuyper F, de Braekeleer J
Arch Virol. 1978;58(3):253-8. doi: 10.1007/BF01317608.
A simple solid phase enzyme immunoassay for the detection of immunoglobulin G and M to cytomegalovirus (CMV) is described. Using this test IgM antibodies to CMV were detected in 0.7 per cent of newborns and regularly after CMV infection in transplant patients, furthermore in these latter patients IgM production was prolonged for several months. For the determination of IgG the enzyme immunoassay was more sensitive than the complement fixation test (CF) and the antibody titres were 4 to 8 fold higher. Since the ELISA test is rapid, specific and unexpensive it can become an acceptable routine diagnostic procedure.
本文描述了一种用于检测巨细胞病毒（CMV）免疫球蛋白G和M的简单固相酶免疫测定法。使用该检测方法，在0.7%的新生儿中检测到了抗CMV的IgM抗体，并且在移植患者感染CMV后可定期检测到；此外，在这些患者中，IgM产生会持续延长数月。对于IgG的测定，酶免疫测定法比补体结合试验（CF）更敏感，抗体滴度高出4至8倍。由于酶联免疫吸附测定（ELISA）试验快速、特异且成本低廉，它可以成为一种可接受的常规诊断方法。
