Kang Jun-Gu, Oh Sung-Suck, Jo Young-Sun, Chae Jeong-Byoung, Cho Yoon-Kyoung, Chae Joon-Seok
1 Laboratory of Veterinary Internal Medicine, BK21 Plus Program for Creative Veterinary Science Research, Research Institute of Veterinary Science and College of Veterinary Medicine, Seoul National University , Seoul, Republic of Korea.
2 Incheon Metropolitan City Institute of Health and Environment , Incheon, Republic of Korea.
Vector Borne Zoonotic Dis. 2018 Aug;18(8):450-452. doi: 10.1089/vbz.2018.2310. Epub 2018 Jun 12.
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne virus in China, Japan, and Korea. Although the incidence of SFTS has been increasing annually since the first SFTSV case was reported in Korea, the prevalence of SFTSV in Korean livestock has not conducted. The purpose of this study was to investigate the prevalence of SFTSV in domesticated pigs (Sus scrofa domesticus) throughout Korea.
A total of 240 sera were collected from 30 domesticated pigs in eight provinces. To detect SFTSV, one-step reverse transcription RT nested PCR was conducted using SFTSV genome-specific primer sets. The amplified PCR products were cloned and then sequenced.
Four of 240 (1.7%) pig sera tested positive for SFTSV through one-step RT nested PCR. Two of the four obtained sequences were included in the Japanese clade, whereas the other two sequences were related to the Chinese clade based on phylogenetic analysis. Moreover, the obtained SFTSV sequences from this study were distinct from human SFTSV strains.
This study is the first to attempt the identification of SFTSV sequences from domesticated pigs and the first molecular detection of SFTSV in Korean livestock. Our findings indicate that a new subclade of SFTSV-different from that in humans-may be present in domesticated pigs; the surveillance of SFTSV in livestock is required to better understand the life cycle of SFTSV.
发热伴血小板减少综合征病毒（SFTSV）是一种在中国、日本和韩国新出现的蜱传病毒。尽管自韩国报告首例SFTSV病例以来，SFTS的发病率逐年上升，但尚未对韩国家畜中SFTSV的流行情况进行调查。本研究的目的是调查韩国各地家猪（Sus scrofa domesticus）中SFTSV的流行情况。
从八个省份的30头家猪中采集了总共240份血清。为了检测SFTSV，使用SFTSV基因组特异性引物对进行一步反转录RT巢式PCR。对扩增的PCR产物进行克隆并测序。
通过一步RT巢式PCR检测，240份猪血清中有4份（1.7%）SFTSV呈阳性。根据系统发育分析，获得的四个序列中有两个属于日本分支，而另外两个序列与中国分支相关。此外，本研究获得的SFTSV序列与人类SFTSV毒株不同。
本研究首次尝试从家猪中鉴定SFTSV序列，并首次在韩国家畜中对SFTSV进行分子检测。我们的研究结果表明，家猪中可能存在一种与人类不同的SFTSV新亚分支；需要对家畜中的SFTSV进行监测，以更好地了解SFTSV的生命周期。
