Felsenstein D, Carney W P, Iacoviello V R, Hirsch M S
J Infect Dis. 1985 Jul;152(1):198-203. doi: 10.1093/infdis/152.1.198.
The phenotypic properties of the surface of the atypical lymphocytes seen in cytomegalovirus-induced (CMV) mononucleosis were evaluated. Mononuclear cells from peripheral blood were obtained from adult patients within seven to 35 days of the onset of acute CMV mononucleosis. Sheep red blood cell rosetting techniques were used to obtain populations depleted of, or enriched for, T cells. Cell populations were further purified for helper/inducer lymphocytes (T4), cytotoxic/suppressor lymphocytes (T8), or non-T-lymphocytes by monoclonal-antibody binding and by complement-lysis techniques. Cytocentrifuge preparations of the cell fractions were evaluated and atypical lymphocytes were identified by morphological characteristics. The appearance and disappearance of atypical lymphocytes paralleled and antedated the increase and subsequent decrease in T8 cells seen in these patients. A total of 69% +/- 22% of the atypical lymphocytes in the lymphocyte population were of the T8 phenotype, whereas 13% +/- 10% of the atypical lymphocytes were of the T4 phenotype and 18% +/- 13% were non-T lymphocytes. Thus, atypical lymphocytes in CMV mononucleosis reside predominantly, but not exclusively, in the T8 cell population.
对巨细胞病毒（CMV）感染所致单核细胞增多症中所见非典型淋巴细胞表面的表型特性进行了评估。从急性CMV单核细胞增多症发病后7至35天内的成年患者获取外周血单个核细胞。采用绵羊红细胞玫瑰花结技术获得T细胞耗竭或富集的细胞群体。通过单克隆抗体结合和补体溶解技术，进一步纯化辅助/诱导淋巴细胞（T4）、细胞毒性/抑制淋巴细胞（T8）或非T淋巴细胞的细胞群体。对细胞组分的细胞离心涂片制备物进行评估，并根据形态学特征鉴定非典型淋巴细胞。非典型淋巴细胞的出现和消失与这些患者中T8细胞的增加及随后的减少同时发生且早于其出现。淋巴细胞群体中总共69%±22%的非典型淋巴细胞为T8表型，而13%±10%的非典型淋巴细胞为T4表型，18%±13%为非T淋巴细胞。因此，CMV单核细胞增多症中的非典型淋巴细胞主要但并非全部存在于T8细胞群体中。
