Farmani Mostafa, Basseri Hamidreza, Norouzi Behzad, Gholizadeh Saber
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran.
BMC Res Notes. 2019 Jan 23;12(1):53. doi: 10.1186/s13104-019-4089-3.
The current study was conducted to identify cockroach species (Blattodea) of northwestern Iran in public places using morphological characteristics and ribosomal DNA internal transcribed spacer 2 (rDNA-ITS2). Sequences were analyzed with Basic Local Alignment Search Tool (BLAST) searches, Neighbor-Joining methods based on and Tamura-Nei phylogenetic analyses. In addition, eight cockroach rDNA-ITS2 sequences from China, India, Iran and the United States obtained from GenBank were compared to those obtained in this study.
Specimens collected in Iran were identified as Periplaneta americana (L.), Shelfordella lateralis (Walker), Blatta orientalis (L.) (Blattodea: Blattidae), Blattella germanica (L.), Supella longipalpa (F.) (Blattodea: Ectobiidae), Polyphaga aegyptiaca (L.), and Polyphaga saussurei (Dohrn) (Blattodea: Corydiidae). rDNA-ITS2 nucleotide sequence analysis showed 100% similarity between P. aegyptiaca and P. saussurei species collected from Iran despite morphological differences. However, ITS2 sequence of P. americana submitted from China showed 30.49-31.71% difference to P. americana sequences from Iran and the United States. The results highlight the importance of morphological identification of cockroach species before conducting molecular techniques.
本研究旨在利用形态特征和核糖体DNA内转录间隔区2（rDNA-ITS2）鉴定伊朗西北部公共场所的蟑螂种类（蜚蠊目）。使用基本局部比对搜索工具（BLAST）搜索、基于邻接法的方法以及塔穆拉-内伊系统发育分析对序列进行分析。此外，还将从GenBank获得的来自中国、印度、伊朗和美国的8条蟑螂rDNA-ITS2序列与本研究中获得的序列进行了比较。
在伊朗采集的标本被鉴定为美洲大蠊（L.）、侧扁大蠊（Walker）、东方蜚蠊（L.）（蜚蠊目：蜚蠊科）、德国小蠊（L.）、长翅迅足长蠊（F.）（蜚蠊目：姬蠊科）、埃及多食蜚蠊（L.）和索氏多食蜚蠊（Dohrn）（蜚蠊目：光蠊科）。rDNA-ITS2核苷酸序列分析表明，尽管形态上存在差异，但从伊朗采集的埃及多食蜚蠊和索氏多食蜚蠊物种之间的相似度为100%。然而，从中国提交的美洲大蠊的ITS2序列与来自伊朗和美国的美洲大蠊序列存在30.49 - 31.71%的差异。结果突出了在进行分子技术之前对蟑螂种类进行形态鉴定的重要性。
