Krönke M, Leonard W J, Depper J M, Greene W C
J Exp Med. 1985 Jun 1;161(6):1593-8. doi: 10.1084/jem.161.6.1593.
Nuclear transcription assays were performed with isolated nuclei from human peripheral blood T lymphocytes stimulated with phytohemagglutinin and phorbol myristate acetate to determine the kinetics of transcriptional activity of various genes occurring in T cell activation. Although silent in resting T cells, the genes encoding c-myc and the interleukin 2 (IL-2) receptor were induced early, preceding gamma interferon (IFN-gamma), IL-2, and transferrin receptor gene transcription. Transcriptional activity of these genes fell after their respective peaks, indicating that the expression of these genes is a transient event during T cell activation. With the exception of the transferrin receptor gene, the kinetics of induction of these genes were not altered by concentrations of cycloheximide that inhibited protein synthesis. These data indicate that the induction of genes encoding c-myc, IL-2, IL-2 receptor, and IFN-gamma occur independently of the sequential production of the proteins they encode.
利用从经植物血凝素和佛波酯刺激的人外周血T淋巴细胞中分离出的细胞核进行核转录分析，以确定T细胞活化过程中各种基因转录活性的动力学。编码c-myc和白细胞介素2（IL-2）受体的基因在静息T细胞中是沉默的，但在γ干扰素（IFN-γ）、IL-2和转铁蛋白受体基因转录之前就被早期诱导。这些基因的转录活性在各自达到峰值后下降，表明这些基因的表达是T细胞活化过程中的一个短暂事件。除转铁蛋白受体基因外，抑制蛋白质合成的环己酰亚胺浓度并未改变这些基因的诱导动力学。这些数据表明，编码c-myc、IL-2、IL-2受体和IFN-γ的基因的诱导独立于它们所编码蛋白质的顺序产生。
