人神经母细胞瘤组织中的肾素和血管紧张素转换酶

Renin and angiotensin-converting enzyme in human neuroblastoma tissue.

作者信息

Mizuno K, Ojima M, Hashimoto S, Fukuchi S

出版信息

J Neurochem. 1985 Aug;45(2):626-9. doi: 10.1111/j.1471-4159.1985.tb04032.x.

DOI:10.1111/j.1471-4159.1985.tb04032.x

Abstract

High activity of renin was demonstrated in human neuroblastoma tissue. This activity was inhibited by specific antibody raised against human renal renin, indicating that it was not due to the nonspecific action of proteases. The specific activity of renin was 122.8 ng of angiotensin I generated mg of protein-1 h-1. It shared some biochemical features with well-known kidney renin, such as molecular weight, optimum pH, the presence of trypsin-activatable inactive renin, and glycoprotein nature. Furthermore, angiotensin-converting enzyme (ACE) activity (2.64 nmol mg of protein-1 min-1) was found in the tissue. This activity was inhibited by captopril, a specific ACE inhibitor, or by omission of chloride ion. These results suggest that true renin in addition to ACE exists in human neuroblastoma tissue.

摘要

在人类神经母细胞瘤组织中证实了肾素的高活性。这种活性被针对人肾肾素产生的特异性抗体所抑制，表明它不是由于蛋白酶的非特异性作用。肾素的比活性为每毫克蛋白质每小时产生122.8纳克血管紧张素I。它与众所周知的肾脏肾素具有一些生化特征，如分子量、最适pH值、存在可被胰蛋白酶激活的无活性肾素以及糖蛋白性质。此外，在该组织中发现了血管紧张素转换酶（ACE）活性（每毫克蛋白质每分钟2.64纳摩尔）。这种活性被特异性ACE抑制剂卡托普利或通过省略氯离子所抑制。这些结果表明，除了ACE之外，人类神经母细胞瘤组织中还存在真正的肾素。