Department of Radiology, The First Affiliated Hospital, Zhengzhou University, No. 1, East Jian She Road, Zhengzhou 450052, Henan Province, People's Republic of China.
Department of Radiology, The First Affiliated Hospital, Zhengzhou University, No. 1, East Jian She Road, Zhengzhou 450052, Henan Province, People's Republic of China.
Eur J Pharmacol. 2018 Aug 15;833:230-236. doi: 10.1016/j.ejphar.2018.06.014. Epub 2018 Jun 9.
Increasing evidences suggested that insufficient radiofrequency ablation (RFA) can paradoxically promote tumor invasion and metastatic processes, while the effects of moderate hyperthermia on cancer progression are not well illustrated. Our present study confirmed moderate hyperthermia treatment can promote the proliferation, migration and invasion of hepatocellular carcinoma (HCC) cells, which was evidenced by the results that moderate hyperthermia induced up regulation of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase-2 (MMP-2). Cellular studies indicated that moderate hyperthermia treatment can increase the mRNA and protein expression of IL-6 and IL-10, while not IL-2, IL-4, IL-8, IL-22, VEGF, TGF-β, or TNF-α, in HCC cells. Silencing of IL-6, while not IL-10, attenuated moderate hyperthermia treatment induced proliferation and cell invasion. Furthermore, our data revealed the inhibition of NF-κB, while not ERK1/2 or PI3K/Akt, abolished moderate hyperthermia treatment induced production of IL-6. Collectively, our data showed that activation of NF-κB/IL-6 is involved in moderate hyperthermia treatment induced progression of HCC cells.
越来越多的证据表明，射频消融（RFA）不足会反常地促进肿瘤侵袭和转移过程，而适度热疗对癌症进展的影响则尚未得到充分说明。我们的研究证实，适度热疗可促进肝癌（HCC）细胞的增殖、迁移和侵袭，这一结果表明适度热疗诱导了增殖细胞核抗原（PCNA）和基质金属蛋白酶-2（MMP-2）的上调。细胞研究表明，适度热疗可增加 HCC 细胞中 IL-6 和 IL-10 的 mRNA 和蛋白表达，但不增加 IL-2、IL-4、IL-8、IL-22、VEGF、TGF-β或 TNF-α的表达。沉默 IL-6 而不是 IL-10 可减弱适度热疗诱导的增殖和细胞侵袭。此外，我们的数据显示，NF-κB 的抑制作用，而不是 ERK1/2 或 PI3K/Akt 的抑制作用，可消除适度热疗诱导的 IL-6 产生。总之，我们的数据表明，NF-κB/IL-6 的激活参与了适度热疗诱导的 HCC 细胞进展。
